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Thermostable collagen-digesting enzyme, novel microorganism producing the enzyme and process for producing the enzyme

Foreign code F040000389
File No. A071-13US
Posted date Feb 22, 2005
Country United States of America
Application number 80774501
Gazette No. 6465236
Date of filing Apr 18, 2001
Gazette Date Oct 15, 2002
International application number PCT/JP1999/006392
International publication number WO 2001/016302
Date of international filing Nov 16, 1999
Date of international publication Mar 8, 2001
Priority data
  • P1999-242816 (Aug 30, 1999) JP
Title Thermostable collagen-digesting enzyme, novel microorganism producing the enzyme and process for producing the enzyme
Abstract Bacillus sp. NTAP-1 having been deposited under accession number FERM BP-6926; and a collagen-decomposing enzyme produced by bacterium. The above enzyme (1) has a capability of hydrolyzing, at the highest efficiency, collagen and gelatin from among casein, gelatin, albumin and collagen; (2) shows the optimum pH of 3.5 to 4.5; (3) shows the optimum temperature of 65 to 70° C.; (4) after heating at 60° C. at pH 6.0 for 4 hours, sustains an activity amounting to 60% or more of the level before the heat treatment; (5) remains stable at pH 3 to 6; and a molecular weight of approximately 46,000 when measured by SDS-PAGE.
  • Inventor, and Inventor/Applicant
  • Nishino; Tokuzo
  • Nakayama; Toru
  • Tsuruoka; Naoki
  • Akai; Minoru
  • Japan Science and Technology Corporation
IPC(International Patent Classification)
Reference ( R and D project ) CREST Social Systems for Better Environment Performance AREA
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