TRANSLATION REGULATION SYSTEM IN CELL OR ARTIFICIAL CELL MODEL BY USING LOW-MOLECULAR-WEIGHT RNA

• 特願2007-303661 (2007.11.22) JP

An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein. The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5' to the ribosome-binding site, and a nucleotide sequence located 5' to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site

an mRNA comprising a small RNA-binding site located 3' to the start codon, and a nucleotide sequence located 3' to the small RNA-binding site, the nucleotide sequence encoding a protein

and a liposome comprising any of these mRNAs encapsulated therein.

• JAPAN SCIENCE AND TECHNOLOGY AGENCY

• INOUE, TAN
• SAITO, HIROHIDE
• KATO, YUSHO
• YOSHIKAWA, KENICHI
• YAMADA, AYAKO
• YAMANAKA, TORU

• C12N  15/09      組換えＤＮＡ技術
• C12N  15/11      ＤＮＡまたはＲＮＡフラグメント；その修飾物
• C12N  15/63      ベクターを用いた外来遺伝物質の導入；ベクター；そのための宿主の使用；発現の制御
• C12P  21/02      ２以上のアミノ酸の結合順序が既知のもの，例．グルタチオン