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Protein-responsive translation control system utilizing rna-protein interaction motif

Foreign code F110003968
File No. I029P004WO
Posted date Jul 7, 2011
Country EPO
Application number 08851746
Gazette No. 2221367
Gazette No. 2221367
Date of filing Nov 21, 2008
Gazette Date Aug 25, 2010
Gazette Date Jul 12, 2017
International application number JP2008071213
International publication number WO2009066757
Date of international filing Nov 21, 2008
Date of international publication May 28, 2009
Priority data
  • P2007-303662 (Nov 22, 2007) JP
  • P2008-186385 (Jul 17, 2008) JP
  • 2008WO-JP71213 (Nov 21, 2008) WO
Title Protein-responsive translation control system utilizing rna-protein interaction motif
Abstract (EP2221367)
An object of the present invention is to provide a translationally regulatable mRNA which has wider application and can perform specific ON-OFF regulation, an RNA-protein complex specifically bound to the mRNA, and a translational regulatory system.
The present invention provides an mRNA having an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5' region of the open reading frame, and an mRNA having a nucleotide sequence complementary to an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5' region of the open reading frame.
Scope of claims [claim1]
1. An mRNA having an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5' region of an open reading frame.
[claim2]
2. An mRNA having a nucleotide sequence complementary to an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5' region of an open reading frame.
[claim3]
3. The mRNA according to claim 1 or 2, wherein the interacting motif is an L7Ae-derived nucleotide sequence.
[claim4]
4. The mRNA according to claim 1 or 2, wherein the interacting motif is a threonyl-tRNA synthetase (ThrRS)-derived nucleotide sequence.
[claim5]
5. An RNA-protein complex comprising an mRNA according to claim 1 and a protein specifically binding to the nucleotide sequence.
[claim6]
6. A translational regulatory system comprising an mRNA according to claim 1 and a protein specifically binding to the nucleotide sequence.
[claim7]
7. A method for translational regulation of mRNA, comprising contacting an mRNA according to claim 1 with a protein specifically binding to the protein-binding motif.
[claim8]
8. A translational regulatory system comprising (a) an mRNA having a nucleotide sequence complementary to an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5' region of the open reading frame, (b) an RNA having the RNA-protein complex interacting motif-derived nucleotide sequence, and (c) a protein specifically binding to the RNA (b).
[claim9]
9. An artificial information conversion system which converts input information of an arbitrary substrate protein to output information of an arbitrary target protein.
[claim10]
10. A simultaneous translational regulatory system which regulates the translational repression and activation of different genes using one protein, the system comprising (a) an mRNA having a nucleotide sequence complementary to an RNA-protein complex interacting motif-derived nucleotide sequence 5' to the ribosome-binding site or within the 5' region of the open reading frame, (b) an RNA having the RNA-protein complex interacting motif-derived nucleotide sequence, (c) a protein specifically binding to the RNA (b), and (d) an mRNA having a nucleotide sequence identical to the nucleotide sequence in the RNA (b), 5' to the ribosome-binding site or within the 5' region of the open reading frame, the mRNA encoding a gene different from that encoded by the mRNA (a).
[claim11]
11. A plasmid vector comprising a nucleic acid sequence encoding an mRNA according to claim 1 or 2.
[claim12]
12. An intracellular translational regulatory system comprising
a first plasmid vector comprising a nucleic acid sequence encoding an mRNA according to claim 1, and
a second plasmid vector comprising a nucleic acid sequence encoding a protein specifically binding to the RNA-protein complex interacting motif-derived nucleotide sequence in the mRNA produced by the first vector.
[claim13]
13. The system according to claim 12, for regulating protein translation in a human cancer cell.
[claim14]
14. A translational regulatory system comprising a fusion protein containing L7Ae as a tag sequence and a first protein.
[claim15]
15. The translational regulatory system according to claim 14, further comprising an mRNA having a sequence specifically binding to L7Ae and a sequence encoding a second protein.
[claim16]
16. An intracellular translational regulatory system comprising a plasmid vector containing a nucleic acid sequence encoding an mRNA encoding L7Ae and a first protein.
[claim17]
17. The intracellular translational regulatory system according to claim 16, further comprising a plasmid vector containing a nucleic acid sequence encoding an mRNA having a sequence specifically binding to L7Ae, the mRNA encoding a second protein.
  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • INOUE TAN
  • SAITO HIROHIDE
  • KOBAYASHI TETSUHIRO
  • HARA TOMOAKI
IPC(International Patent Classification)
U.S. Cl./(Sub)
  • C12N015/67
Specified countries (EP2221367)
Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR
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