Top > Search of International Patents > METHOD FOR ENRICHMENT AND/OR ISOLATION OF DOPAMINERGIC NEURONS

METHOD FOR ENRICHMENT AND/OR ISOLATION OF DOPAMINERGIC NEURONS meetings achieved

Foreign code F110004218
File No. A011-09CA
Posted date Jul 12, 2011
Country Canada
Application number 2381065
Gazette No. 2381065
Gazette No. 2381065
Date of filing Dec 7, 2000
Gazette Date Dec 6, 2001
Gazette Date Jun 5, 2007
Priority data
  • P2000-165150 (Jun 1, 2000) JP
Title METHOD FOR ENRICHMENT AND/OR ISOLATION OF DOPAMINERGIC NEURONS meetings achieved
Abstract

A method which comprises transferring reporter nucleic acid molecules, which express a fluorescent protein under the regulation by the promoter/enhancer of a gene expressed in dopaminergic neurons, into individual cells of a cell ma ss and separating fluorescing cells from this cell mass. A method which compris es transferring the above-described reporter nucleic acid molecules into individual cells of a cell mass and measuring the fluorescence distribution of the cell mass to thereby visualize and identify the dopaminergic neurons occurring in the cell mass in a viable state.

A method of identifying a dopaminergic neuron inductive factor which comprises transferring a reporter nucleic acid molecule into cells capable of differentiating into dopaminergi c neurons, allowing these cells to coexist with a candidate substance and then determining whether or not the candidate substance is a dopaminergic neuron inductive factor with the use of the fluorescence of the cells as an indication.

  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY CORPORATION
  • Inventor
  • MATSUSHITA, NATSUKI,
  • KOBAYASHI, KAZUTO,
  • OKANO, HIDEYUKI,
  • SAWAMOTO, KAZUNOBU
IPC(International Patent Classification)
Reference ( R and D project ) CREST Understanding the Brain AREA
Please contact us by E-mail or facsimile if you have any interests on this patent.

PAGE TOP

close
close
close
close
close
close