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PRODUCTION OF HYBRIDOMA PRODUCING ANTIHUMAN CENP-A PEPTIDE MONOCLONAL ANTIBODY AND METHOD OF USING THE SAME achieved

Foreign code F110004240
File No. A021-08CA
Posted date Jul 13, 2011
Country Canada
Application number 2474880
Gazette No. 2474880
Gazette No. 2474880
Date of filing Jul 4, 2002
Gazette Date Aug 7, 2003
Gazette Date Nov 24, 2009
Priority data
  • P2002-023587 (Jan 31, 2002) JP
Title PRODUCTION OF HYBRIDOMA PRODUCING ANTIHUMAN CENP-A PEPTIDE MONOCLONAL ANTIBODY AND METHOD OF USING THE SAME achieved
Abstract

It is intended to provide antibodies, in particular, monoclonal antibodies against, as antigens, peptides comprising N-terminal 1/3 amino acid sequences of a protein CENP-A expressed in centromeres in chromosome and a process for producing th e same. Antibodies against partial-length peptides of the CENP-A protein expressed in centromeres in chromosome (preferably a peptide having the amino acid sequen ce of 3- to 19-positions of the CENP-A protein, a peptide having the amino acid sequence of 27- to 40-positions thereof or a peptide having the amino acid sequence of 1 26- to 140-positions thereof)

and a process for producing the same. A method of solubilizing a centromere chromatin while sustaining its completeness by treating with 0.3 M NaCl and MNase.

A method of using an anti-CENP-A antibody which comprises chemically bonding the antibody to a support and isolating and purifying the same by the immunoprecipitation method.

  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • OKAZAKI, TSUNEKO,
  • YODA, KINYA,
  • NOZAKI, NAOHITO
IPC(International Patent Classification)
Reference ( R and D project ) CREST Genetic Programming AREA
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