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MAMMALIAN ARTIFICIAL CHROMOSOME achieved

Foreign code F110004243
File No. A022-23CA
Posted date Jul 13, 2011
Country Canada
Application number 2501708
Gazette No. 2501708
Gazette No. 2501708
Date of filing Sep 1, 2003
Gazette Date Mar 18, 2004
Gazette Date Jan 3, 2012
Priority data
  • P2002-258114 (Sep 3, 2002) JP
  • P2002-338865 (Nov 22, 2002) JP
Title MAMMALIAN ARTIFICIAL CHROMOSOME achieved
Abstract

It is intended to provide an artificial mammalian chromosome which is stably held in mammalian cells and allows efficient expression of a target gene carried thereby. Namely, a first cyclic vector containing a mammalian centromere sequence and a selection marker gene and a second cyclic vector containing a functional sequence are transferred into mammalian host cells. Then transformed cells are selected by using the above-described selection marker gene and cells holding an artificial mammalian chromosome are selected from among the transformed cells thus selected. Thus, it is possible to construct an artificial mammalian chromosome which has a mammalian replication origin, the mammalian centromere sequence and the functional sequence, is in a cyclic form, can be replicated in mammalian cells, extrachromosomally held in the host cells and transferred to daughter cells in cell division.

  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • IKENO, MASASHI,
  • OKAZAKI, TSUNEKO,
  • SUZUKI, NOBUTAKA,
  • ITOU, TOSHIHIDE
IPC(International Patent Classification)
Reference ( R and D project ) CREST Genetic Programming AREA
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