BACTERIAL CELL COMPONENT-UNRESPONSIVE MODEL MOUSE
|Posted date||Jul 13, 2011|
|Date of filing||Jan 13, 2000|
|Gazette Date||Jul 20, 2000|
|Gazette Date||Aug 25, 2009|
|Title||BACTERIAL CELL COMPONENT-UNRESPONSIVE MODEL MOUSE|
A knockout mouse which is unresponsive to peptidoglycan, a lipoprotein/lipopeptide and the like, and is useful for elucidating the contribution of individual members of the TLR family to a signaling stimulated with bacterial cell components in vivo, in particular, the role of TLR2 and MyD88 in vivo. A bacterial cell component-unresponsive knockout mouse is generated by a process comprising the steps of: a targeting vector is constructed by replacing a whole or a part of a gene fragment of an exon region containing a cytoplasmic region of TLR2 or MyD88 gene and the like with a plasmid having a poly A signal and a marker gene
the targeting vector is introduced into an embryonic stem cell
the targeting embryonic stem cell having a homologously recombined TLR2 or MyD88 gene is microinjected into the blastocyst of a mouse and the blastocyst is put back into the uterus of a recipient mouse.
|IPC(International Patent Classification)||
|Reference ( R and D project )||CREST Host Defense Mechanism AREA|
Contact Information for " BACTERIAL CELL COMPONENT-UNRESPONSIVE MODEL MOUSE "
- Japan Science and Technology Agency Department of Intellectual Property Management
- URL: http://www.jst.go.jp/chizai/
- Address: 5-3, Yonbancho, Chiyoda-ku, Tokyo, Japan , 102-8666
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