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BACTERIAL CELL COMPONENT-UNRESPONSIVE MODEL MOUSE achieved

Foreign code F110004247
File No. A031-19CA
Posted date Jul 13, 2011
Country Canada
Application number 2368217
Gazette No. 2368217
Gazette No. 2368217
Date of filing Jan 13, 2000
Gazette Date Jul 20, 2000
Gazette Date Aug 25, 2009
Priority data
  • P1999-007365 (Jan 14, 1999) JP
  • P1999-228282 (Aug 12, 1999) JP
  • P1999-309238 (Oct 29, 1999) JP
Title BACTERIAL CELL COMPONENT-UNRESPONSIVE MODEL MOUSE achieved
Abstract

A knockout mouse which is unresponsive to peptidoglycan, a lipoprotein/lipopeptide and the like, and is useful for elucidating the contribution of individual members of the TLR family to a signaling stimulated with bacterial cell components in vivo, in particular, the role of TLR2 and MyD88 in vivo. A bacterial cell component-unresponsive knockout mouse is generated by a process comprising the steps of: a targeting vector is constructed by replacing a whole or a part of a gene fragment of an exon region containing a cytoplasmic region of TLR2 or MyD88 gene and the like with a plasmid having a poly A signal and a marker gene

the targeting vector is introduced into an embryonic stem cell

the targeting embryonic stem cell having a homologously recombined TLR2 or MyD88 gene is microinjected into the blastocyst of a mouse and the blastocyst is put back into the uterus of a recipient mouse.

  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY CORPORATION
  • Inventor
  • TAKEDA, KIYOSHI,
  • AKIRA, SHIZUO,
  • TAKEUCHI, OSAMU
IPC(International Patent Classification)
Reference ( R and D project ) CREST Host Defense Mechanism AREA
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