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DNA FRAGMENT, METHOD FOR PRODUCTING TRANSFORMANT FOR PRODUCING TRANSFORMANT FOR PROTEIN PRODUCTION AND UTILIZATION THEREOF

Foreign code F110004322
File No. A181-07WO
Posted date Jul 13, 2011
Country Canada
Application number 2540668
Gazette No. 2540668
Gazette No. 2540668
Date of filing Oct 1, 2004
Gazette Date Apr 14, 2005
Gazette Date Dec 21, 2010
Priority data
  • P2003-343747 (Oct 1, 2003) JP
  • P2003-350091 (Oct 8, 2003) JP
  • P2004-056912 (Mar 1, 2004) JP
Title DNA FRAGMENT, METHOD FOR PRODUCTING TRANSFORMANT FOR PRODUCING TRANSFORMANT FOR PROTEIN PRODUCTION AND UTILIZATION THEREOF
Abstract

An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein of ToMV having a suppressor for a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cell, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.

  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • TAMAI, ATSUSHI,
  • DOHI, KOJI,
  • NISHIKIORI, MASAKI,
  • ISHIKAWA, MASAYUKI,
  • MORI, MASASHI,
  • MESHI, TETSUO
IPC(International Patent Classification)
Reference ( R and D project ) CREST Plants Function and Their Control AREA
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