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NONHUMAN MODEL ANIMAL UNRESPONSIVE TO IMMUNOPOTENTIATING SYNTHETIC COMPOUND achieved

Foreign code F110004379
File No. B02-01CA
Posted date Jul 14, 2011
Country Canada
Application number 2468011
Gazette No. 2468011
Gazette No. 2468011
Date of filing Nov 22, 2002
Gazette Date May 30, 2003
Gazette Date Apr 10, 2012
Priority data
  • P2001-358295 (Nov 22, 2001) JP
Title NONHUMAN MODEL ANIMAL UNRESPONSIVE TO IMMUNOPOTENTIATING SYNTHETIC COMPOUND achieved
Abstract

It is intended to provide a nonhuman model animal unresponsive to a synthetic compound which lacks a gene function encoding TLR7 recognizing an immunopotentiating synthetic compound such as imidazoquinoline on its chromosome. A gene fragment of the whole gene sites including the intracellular domain and the transmembrane domain or a part thereof of a TLR7 gene obtained from a mouse gene library is substituted by a plasmid having polyA signal and a marker gene to construct a targeting vector. Then this targeting vector is linearized and transferred into embryo stem cells. The target embryo stem cells lacking the TLR7 gene function are microinjected into a mouse blastocyst to construct a chimeric mouse. Then this chimeric mouse is crossed with a wild type mouse to give a heterozygote mouse. Next, the heterozygote mouse is intercrossed and thus a TLR7 knockout mouse is obtained.

  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY,
  • AKIRA, SHIZUO,
  • SUMITOMO PHARMACEUTICALS COMPANY LIMITED
  • Inventor
  • YAMAOKA, TAKASHI,
  • TOMIZAWA, HIDEYUKI,
  • AKIRA, SHIZUO
IPC(International Patent Classification)
Reference ( R and D project ) SORST Selected in Fiscal 2000
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