NONHUMAN MODEL ANIMAL UNRESPONSIVE TO IMMUNOPOTENTIATING SYNTHETIC COMPOUND
|Posted date||Jul 14, 2011|
|Date of filing||Nov 22, 2002|
|Gazette Date||May 30, 2003|
|Gazette Date||Apr 10, 2012|
|Title||NONHUMAN MODEL ANIMAL UNRESPONSIVE TO IMMUNOPOTENTIATING SYNTHETIC COMPOUND|
It is intended to provide a nonhuman model animal unresponsive to a synthetic compound which lacks a gene function encoding TLR7 recognizing an immunopotentiating synthetic compound such as imidazoquinoline on its chromosome. A gene fragment of the whole gene sites including the intracellular domain and the transmembrane domain or a part thereof of a TLR7 gene obtained from a mouse gene library is substituted by a plasmid having polyA signal and a marker gene to construct a targeting vector. Then this targeting vector is linearized and transferred into embryo stem cells. The target embryo stem cells lacking the TLR7 gene function are microinjected into a mouse blastocyst to construct a chimeric mouse. Then this chimeric mouse is crossed with a wild type mouse to give a heterozygote mouse. Next, the heterozygote mouse is intercrossed and thus a TLR7 knockout mouse is obtained.
|IPC(International Patent Classification)||
|Reference ( R and D project )||SORST Selected in Fiscal 2000|
Contact Information for " NONHUMAN MODEL ANIMAL UNRESPONSIVE TO IMMUNOPOTENTIATING SYNTHETIC COMPOUND "
- Japan Science and Technology Agency Department of Intellectual Property Management
- URL: http://www.jst.go.jp/chizai/
- Address: 5-3, Yonbancho, Chiyoda-ku, Tokyo, Japan , 102-8666
- Fax: 81-3-5214-8476