AMPLIFYING ATP WITH ADP-FREE POLYPHOSPHATE-ADENYLATE KINASE FUSION PROTEIN
|Posted date||Jul 15, 2011|
|Date of filing||Jul 27, 2004|
|Gazette Date||Feb 3, 2005|
|Gazette Date||May 11, 2010|
|Title||AMPLIFYING ATP WITH ADP-FREE POLYPHOSPHATE-ADENYLATE KINASE FUSION PROTEIN|
The ATP amplification method of the present invention is a method for amplifying and detecting a very trace amount of exogenous ATP by allowing a fusion protein (PPK-ADK) of a polyphosphate kinase and an adenylate kinase, the fusion protein not containing ADP, to act on a mixture of ATP, AMP, and a polyphosphate compound. The present invention also provides an ultrasensitive ATP amplification method by which ATP at a single cell level can be amplified and detected, and an ultrasensitive microbial assay based on this ATP amplification method.
|IPC(International Patent Classification)||
|Reference ( R and D project )||PRESTO Conversion and Control by Advanced Chemistry AREA|
Contact Information for " AMPLIFYING ATP WITH ADP-FREE POLYPHOSPHATE-ADENYLATE KINASE FUSION PROTEIN "
- Japan Science and Technology Agency Department of Intellectual Property Management
- URL: http://www.jst.go.jp/chizai/
- Address: 5-3, Yonbancho, Chiyoda-ku, Tokyo, Japan , 102-8666
- Fax: 81-3-5214-8476