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AMPLIFYING ATP WITH ADP-FREE POLYPHOSPHATE-ADENYLATE KINASE FUSION PROTEIN meetings achieved

Foreign code F110004438
File No. K05323WO
Posted date Jul 15, 2011
Country Canada
Application number 2533440
Gazette No. 2533440
Gazette No. 2533440
Date of filing Jul 27, 2004
Gazette Date Feb 3, 2005
Gazette Date May 11, 2010
Priority data
  • P2003-202992 (Jul 29, 2003) JP
Title AMPLIFYING ATP WITH ADP-FREE POLYPHOSPHATE-ADENYLATE KINASE FUSION PROTEIN meetings achieved
Abstract

The ATP amplification method of the present invention is a method for amplifying and detecting a very trace amount of exogenous ATP by allowing a fusion protein (PPK-ADK) of a polyphosphate kinase and an adenylate kinase, the fusion protein not containing ADP, to act on a mixture of ATP, AMP, and a polyphosphate compound. The present invention also provides an ultrasensitive ATP amplification method by which ATP at a single cell level can be amplified and detected, and an ultrasensitive microbial assay based on this ATP amplification method.

  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • KURODA, AKIO
IPC(International Patent Classification)
Reference ( R and D project ) PRESTO Conversion and Control by Advanced Chemistry AREA
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