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Method of measuring interaction between biomaterial and sugar chain, method of evaluating biomaterial in sugar chain selectivity, method of screening biomaterial, method of patterning biomaterials, and kits for performing these methods

Foreign code F110004468
File No. V350P002CA
Posted date Jul 15, 2011
Country Canada
Application number 2591496
Gazette No. 2591496
Gazette No. 2591496
Date of filing Jun 15, 2007
Gazette Date Jun 18, 2008
Gazette Date Sep 2, 2014
Priority data
  • P2006-340554 (Dec 18, 2006) JP
Title Method of measuring interaction between biomaterial and sugar chain, method of evaluating biomaterial in sugar chain selectivity, method of screening biomaterial, method of patterning biomaterials, and kits for performing these methods
Abstract This invention provides a method of screening or patterning a biomaterial in terms of their specificities to sugar chains by performing real-time and comprehensive measurement of an interaction between sugar chains and the biomaterial concurrently with a very small amount of the biomaterial without labeling.
It is a method of measuring an interaction between a biomaterial and a sugar chain(s), the method including: bringing a solution containing the biomaterial in contact with a ligand carrier, the ligand carrier including a support whose surface includes a metal. and a ligand conjugate(s) immobilized independently on the surface, the ligand carrier carrying the ligand conjugate(s) in such a manner that 1 to 500 kinds of the ligand conjugate(s) is immobilized per cm2. and each ligand conjugate having a structure in which a sugar chain is bonded with a linker compound having a sulfur atom. and the biomaterial being at least one selected from the groups of proteins. viruses, cells, microorganisms, liposome, and micelles.
  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • SUDA YASUO
  • NISHIMURA TOMOAKI
  • KISHIMOTO YUKO
  • YAMASHITA SAKIKO
  • TSURUTA SACHIKO
  • WAKAO MASAHIRO
  • OKUNO TOSHIOMI
IPC(International Patent Classification)
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