Sugar chain ligand composite and method of analyzing protein with the ligand composite
|発明の名称 （英語）||Sugar chain ligand composite and method of analyzing protein with the ligand composite|
A novel ligand composite which is effectively utilizable for analyzing a function of a protein; a ligand- supporting object; and a method of analyzing a protein.
The ligand composite has a structure which comprises: a linker compound having a structure represented by the following general formula (1): (wherein n and p each is an integer of 0 to 6) in which X is a structure comprising one, two, or three hydrocarbon derivative chains which have an aromatic amino group at the end and may have a carbon-nitrogen bond in the main chain, Y is a hydrocarbon structure containing one or more sulfur atoms, and Z is a linear structure comprising a carbon-carbon bond or carbon- oxygen bond; and a sugar which has a reducing end and is bonded to the linker compound through the aromatic amino group.
General formula (1)
(P and q is independent of the an integer between 0 and 6 and below) is represented in the structure with the
Ligand complexes to have joined through the above aromatic amino Saccharides with aromatic amino terminus as above, with linker compounds have a linear chain structure structure may have carbon 1 nitrogen coupling in main-chain hydrocarbon induced chain 1 chain or chain 2 or 3 strands, comprising the above Y containing sulfur atoms or sulfur atoms hydrocarbon hydrogen structure with a carbon one-carbon bond or carbon and one oxygen bond with above Z as a reducing end and structure.
2. Above Y is S ― to claim 1 characterized by hydrocarbon hydrogen structure containing the binding S or S H stated ligand complexes.
3. Above is a general expression (2)
(M1, m2, m3 is independent of the integers between 0 and 6. R ' is hydrogen (H) or R. ) Is represented in the structure with the
These R the sugar chains derived from compounds and special 癍 to claim 1 or 2 stated ligand complexes.
4. the above is general formula (3)
(M-4and m-5 is independent of the integers between 0 and 6. R ' is hydrogen (H) or RD ) represented the structure with the
Above r-derived compounds that characterized a claim 1 or 2 stated ligand complexes.
5. the above is general formula (4)
(The R ' is hydrogen (Η) or R) structure represented by the above R sugar chains derived from claim 1 characterized by compound or 2 stated ligand complexes.
6. above Ζ the expression (5) or (6).
(The n 1, η 2 is an integer for each of the 6) to features and has a structure that is represented by claim 1 or 2 stated ligand complexes.
7. General-earthling 7)
(M1, m2, m3 is independent of the expression in the, an integer between 0 and 6, n 1 is an integer between 1 and 6 or less) has a structure that is represented by the linker compounds or general expressions (8)
Γ (m-4and m-5 is independent of the the η 1 , an integer between 0 and 6 or less more than 6 less than or equal) linker is represented by the structure of compounds 1, also a general expression (9)
' (N 1, q is independent of the integers between 0 and 6) linker compound has a structure and is represented in, or the general formula (1 0)
(The n 2 is an integer between 1 and 6) Li 5 anchors compound has a structure that is represented in, or general expression (1 1)
10 . (The n 1 is an integer between 1 and 6) Li have represented the structure, ' poorly compounds and
, . Characterized by having reducing glucose and through reductive amination-ligand complexes to manufacturing methods.
, 8 . Claim 1 or paragraph (1) or 6, ligand complexes in... surface ^ ligand carriers that onto the substrate with metal peg that characterized.
9. To claim 8 to be used in the analysis of the protein-ligand carriers listed.
1 0. Made the ligand complexes immobilized on support by either claim 1 or 6 to contact stated ligand complexes to support 20 paragraph (1)-ligand carriers when creating and
' After contacted with the protein solution above ligand carriers to the molecular interaction interaction measurement process and the
After the above Intermolecular interaction measurement in mass spectrometry, 2"process to identify the proteins attached to the material and
From which methods for determination of protein to feature.
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