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Biocompatible transparent sheet, method of producing the same and cell sheet

外国特許コード F110004904
整理番号 RJ008P96WO
掲載日 2011年7月22日
出願国 大韓民国
出願番号 20087022611
公報番号 1020090007288
公報番号 101084429
出願日 平成19年3月14日(2007.3.14)
公報発行日 平成21年1月16日(2009.1.16)
公報発行日 平成23年11月21日(2011.11.21)
国際出願番号 JP2007055028
国際公開番号 WO2007108373
国際出願日 平成19年3月14日(2007.3.14)
国際公開日 平成19年9月27日(2007.9.27)
優先権データ
  • 2007JP55028 (2007.3.14) WO
  • 特願2006-074254 (2006.3.17) JP
発明の名称 (英語) Biocompatible transparent sheet, method of producing the same and cell sheet
発明の概要(英語) It is intended to provide a biocompatible transparent sheet which is highly capable of absorbing a biocompatible or biologically-relevant substance and usable as a novel biological material, enables real-time observation of the proliferation and differentiation of living cells and so on and has sufficient flexibility and softness. This biocompatible transparent sheet is produced by forming a biocompatible ceramic film (2) by, for example, the laser ablation method on a base material (1) being soluble in a solvent (11) in which the biocompatible ceramic is insoluble, dipping the base material (1) having the film formed thereon in the solvent (11) to thereby dissolve the base material (1) and then drying the film (2) thus separated. By seeding and growing cells on the surface of this biocompatible transparent sheet (2) having sufficient flexibility and softness, it is possible to construct a cell sheet which can be directly transplanted into an affected part.
(C) KIPO WIPO 2009
従来技術、競合技術の概要(英語) BACKGROUND ART
And the like hydroxyapatite of its biocompatible ceramic is so high that high affinity, this was coated as a thin film of metal or ceramic is good and as a biomaterial, cells on these substrates by culturing the cell proliferation or differentiation of it becomes faster (see Patent document 1) was confirmed. In addition, biocompatible coating ceramic sheets because they have a high adsorption property of a biological substance, such as separation and analysis of a nucleic acid or protein is assembled into sheet or the like has been studied (see Patent document 2 and 3). In addition, living cells in order to observe directly employ, and biocompatible ceramics sintered at high temperatures of the ground to form a transparent body consisting of there bulk below.
In addition to this, the biodegradable polymer and an amphiphilic polymer is a hydrophobic organic solvent solution as the raw material (see Patent document 4) a porous film, is overlaid on a fibrin gel and the like suffering from sheet and an adherent cultured cells using the same (see Patent document 5) of the stratified sheet exists.
However, biocompatible coating ceramic substrate because of the lack of flexibility in a range of use is was limited and, on the thin film of the cells cultured in the desired function is expressed tissue state and there is recovered in, in vivo after transplantation absorbed during the cell sheet that was not can be also prepared. In addition, metals or ceramics substrate may be opaque since the, living cells such as adhesive or differentiation of the behavior of was also cannot be observed in real time.
In addition, biocompatible coating ceramic sheets can be separated from the biological substance adsorption b·s′ may return, an opaque sheet since the interaction between the biological substance or the like could not be observed in real time.
In addition, biocompatible ceramics of the thinned by polishing the bulk it and there is a limit, which is provided with the transparent body is flexible and flexibility was not obtained. For this reason, a transparent body and the flexibility of the soft tissue and the like tissues in need there was no able to be used as a covering material.
In addition, the porous film is a synthetic polymer resin of an amphiphilic polymer since it contains the toxicity of the living body is reactive and is less likely to occur, adherent cultured cell sheet is suffering from fibrin gel biological substance because of the inclusion of an infection of the virus or the like was less likely to occur.
Patent document 1: Japanese Unexamined patent application publication 2005-278609 publication
Patent document 2: Japanese Patent laid open publication (hei) No. 07-88819 propanedini
Patent document 3: Japanese Patent Application Laid open Publication (hei) No. 10-156814
Patent document 4: Japanese Laid-Open Patent Application Publication No. 2001-157574
Patent document 5: Japanese Laid-Open Patent Application Publication No. 2005-608
特許請求の範囲(英語) [claim1]
1. Biocompatible ceramic being soluble in a solvent which does not dissolve a portion of the substrate comprising, biocompatible ceramics film is deposited film formation step, and
Biocompatible ceramic film is formed by immersing the substrate with the solvent dissolving the substrate melting step of
Comprising a method of manufacturing a biocompatible transparent sheet.

[claim2]
2. Method according to claim 1, wherein the substrate is being soluble in a solvent portion and is constituted by a portion insoluble in a solvent in which the method of manufacturing a biocompatible transparent sheet.

[claim3]
3. Method according to claim 1, the laser deposition method, sputtering method, an ion beam deposition method, an electron beam deposition method, a vacuum evaporation method, molecular beam epitaxy method, a chemical vapor deposition method by the method of any one of the film forming biocompatible biocompatible ceramics manufacturing method of the transparent sheet.

[claim4]
4. Method according to claim 1, after dissolving the substrate, the biocompatible ceramic film isolated comprising a drying step of drying a biocompatible transparent sheet.

[claim5]
5. Method according to claim 1, the film forming biocompatible or after the drying the ceramic, high temperature water vapor containing gas and carbon dioxide containing gas in an atmosphere comprising at least one of heat treatment comprising the steps of the method of manufacturing a biocompatible transparent sheet.

[claim6]
6. Method according to claim 1, biocompatible hydroxyapatite ceramic membrane composed of a biocompatible transparent sheet.

[claim7]
7. Method according to claim 1, different types of biocompatible ceramics film is composed of a plurality of apatite biocompatible transparent sheet.

[claim8]
8. 1 the first method obtained by the method for producing biocompatible transparent sheet.

[claim9]
9. Method according to claim 8, wherein a thickness of 1 to 100 micro M biocompatible transparent sheet.

[claim10]
10. Method according to claim 8, upper and lower surfaces having through holes penetrating between the biocompatible transparent sheet.

[claim11]
11. Method according to claim 8, composed of hydroxyapatite biocompatible transparent sheet.

[claim12]
12. Method according to claim 8, a plurality of different types of biocompatible ceramics composed of a biocompatible transparent sheet.

[claim13]
13. 8 the first method for growing cells on the biocompatible transparent sheet obtained by cell sheet.

[claim14]
14. AMEND STATUS: Delete

[claim15]
15. AMEND STATUS: delete

[claim16]
16. AMEND STATUS: delete

[claim17]
17. AMEND STATUS: delete
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • KINKI UNIVERSITY
  • 発明者(英語)
  • HONTSU SHIGEKI
  • NISHIKAWA HIROAKI
  • KUSUNOKI MASANOBU
  • HATANAKA RYOUTA
国際特許分類(IPC)
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