CYTODETACHING AGENT AND METHOD OF DETACHING CELL SHEET
A cell sheet detaching agent comprising an aminated polyrotaxane. The polyrotaxane constituting the skeleton of this cytodetaching agent has such a structure that multiple cyclic molecules are arranged so as to have a linear molecule pass therethrough, the linear molecule at both ends thereof having bulky caps bonded thereto so as to prevent dropping of the cyclic molecules therefrom. The aminated polyrotaxane contained in the cytodetaching agent is a compound resulting from replacement of at least some of hydroxyls of the cyclodextrin skeleton contained in the polyrotaxane by an amino-having substituent. By the use of this cell sheet detaching agent, cultured cells adhering to a container surface can be detached without causing any cell damaging and without the need to conduct temperature control.