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Transgenic toll-like receptor 9 (TLR9) mice achieved

Foreign code F110005032
File No. A031-29US
Posted date Aug 18, 2011
Country United States of America
Application number 08856701
Gazette No. 20030124655
Gazette No. 7507872
Date of filing Jun 5, 2001
Gazette Date Jul 3, 2003
Gazette Date Mar 24, 2009
International application number JP2001004731
International publication number WO2002006482
Date of international filing Jun 5, 2001
Date of international publication Jan 24, 2002
Priority data
  • P2000-219652 (Jul 19, 2000) JP
  • 2001WO-JP04731 (Jun 5, 2001) WO
Title Transgenic toll-like receptor 9 (TLR9) mice achieved
Abstract (US7507872)
The present invention provides a receptor protein specifically recognizing bacterial DNA having an unmethylated CpG sequence, a genomic DNA encoding it, an experimental animal model useful for examining responsiveness of a host immune cell against a bacterial infectious disease.
DNA encoding a receptor protein specifically recognizing bacterial DNA having an unmethylated CpG sequence is screened by BLAST search, a number of EST clones having high homology with various TLRs is screened, these clones are used as a probe to isolate a full-length cDNA from mouse macrophage cDNA library, and the sequence of bases of the cDNA is analyzed to confirm that it is TLR9 comprising a conserved regions such as LRR and TIR regions, and then a knockout mouse is produced to confirm that TLR9 is a receptor protein of oligonucleotides having an unmethylated CpG sequence of bacterial DNA.
Scope of claims [claim1]
1. A transgenic mouse whose genome comprises a homozygous inactivation of the Toll-like Receptor 9 (TLR9) gene such that no functional N-terminal fragment of TLR9 is produced;
said TLR9 gene encodes a polypeptide that recognizes CpG oligodeoxynucleotide (ODN), wherein macrophages of said mouse exhibit decreased responsiveness to CpG ODN.
[claim2]
2. A cell obtained from the transgenic mouse according to claim 1.
  • Inventor, and Inventor/Applicant
  • AKIRA SHIZUO
  • HEMMI HIROAKI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
Reference ( R and D project ) CREST Host Defense Mechanism AREA
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