Transgenic toll-like receptor 9 (TLR9) mice
|Posted date||Aug 18, 2011|
|Country||United States of America|
|Date of filing||Jun 5, 2001|
|Gazette Date||Jul 3, 2003|
|Gazette Date||Mar 24, 2009|
|International application number||JP2001004731|
|International publication number||WO2002006482|
|Date of international filing||Jun 5, 2001|
|Date of international publication||Jan 24, 2002|
|Title||Transgenic toll-like receptor 9 (TLR9) mice|
The present invention provides a receptor protein specifically recognizing bacterial DNA having an unmethylated CpG sequence, a genomic DNA encoding it, an experimental animal model useful for examining responsiveness of a host immune cell against a bacterial infectious disease.
DNA encoding a receptor protein specifically recognizing bacterial DNA having an unmethylated CpG sequence is screened by BLAST search, a number of EST clones having high homology with various TLRs is screened, these clones are used as a probe to isolate a full-length cDNA from mouse macrophage cDNA library, and the sequence of bases of the cDNA is analyzed to confirm that it is TLR9 comprising a conserved regions such as LRR and TIR regions, and then a knockout mouse is produced to confirm that TLR9 is a receptor protein of oligonucleotides having an unmethylated CpG sequence of bacterial DNA.
|Scope of claims||
1. A transgenic mouse whose genome comprises a homozygous inactivation of the Toll-like Receptor 9 (TLR9) gene such that no functional N-terminal fragment of TLR9 is produced;
said TLR9 gene encodes a polypeptide that recognizes CpG oligodeoxynucleotide (ODN), wherein macrophages of said mouse exhibit decreased responsiveness to CpG ODN.
2. A cell obtained from the transgenic mouse according to claim 1.
|IPC(International Patent Classification)||
|Reference ( R and D project )||CREST Host Defense Mechanism AREA|
Contact Information for " Transgenic toll-like receptor 9 (TLR9) mice "
- Japan Science and Technology Agency Department of Intellectual Property Management
- URL: http://www.jst.go.jp/chizai/
- Address: 5-3, Yonbancho, Chiyoda-ku, Tokyo, Japan , 102-8666
- Fax: 81-3-5214-8476