Top > Search of International Patents > Stable isotope-labeled aromatic amino acids, method for incorporating the same in target protein and method for NMR-structural analysis of proteins

Stable isotope-labeled aromatic amino acids, method for incorporating the same in target protein and method for NMR-structural analysis of proteins achieved

Foreign code F110005215
File No. A202-01WO
Posted date Aug 26, 2011
Country United States of America
Application number 41475606
Gazette No. 20060194328
Gazette No. 07608248
Date of filing Apr 28, 2006
Gazette Date Aug 31, 2006
Gazette Date Oct 27, 2009
International application number PCT/JP2004/016215
Date of international filing Nov 1, 2004
Priority data
  • P2003-373304 (Oct 31, 2003) JP
Title Stable isotope-labeled aromatic amino acids, method for incorporating the same in target protein and method for NMR-structural analysis of proteins achieved
Abstract The present invention herein provides, for instance, a stable isotope-labeled phenylalanine wherein a carbon atom of the phenyl group linked to an amino acid residue is 13C, 2 to 4 carbon atoms of the remaining 5 carbon atoms constituting the phenyl group are 12C atoms to which deuterium atoms are bonded, and the remaining carbon atoms are 13C atoms to which hydrogen atoms are linked, and a stable isotope-labeled tyrosine wherein a carbon atom of the phenyl group linked to an amino acid residue is 13C, the carbon atom bonded to the hydroxyl group (OH group) of the phenyl group is 12C or 13C, 2 to 4 carbon atoms of the remaining 4 carbon atoms constituting the phenyl group are 12C atoms to which deuterium atoms are bonded, and the remaining carbon atoms are 13C atoms to which hydrogen atoms are linked. The stable isotope-labeled amino acid permits the elimination of such a conventional problem concerning the complexity of the NMR signals ascribed to aromatic rings, the complexity being a principal cause of making the NMR analysis difficult, encountered when using the conventional uniformly labeled amino acid residue. Moreover, the isotope-labeled amino acid likewise permits the substantial improvement of the sensitivity thereof to the NMR spectroscopic analysis.
  • Inventor, and Inventor/Applicant
  • Kainosho, Masatsune; Tokyo [JP]
  • Terauchi, Tsutomu; Sagamihara [JP]
  • Japan Science and Technology Agency, Saitama [JP]
IPC(International Patent Classification)
Reference ( R and D project ) CREST Understanding the Brain (Mechanisms of Brain) AREA
Please contact us by E-mail or facsimile if you have any interests on this patent.

PAGE TOP

close
close
close
close
close
close