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Gene carriers with the use of polysaccharide and process for producing the same achieved

Foreign code F110005347
File No. K01202US
Posted date Aug 31, 2011
Country United States of America
Application number 47136602
Gazette No. 20060084149
Gazette No. 7521433
Date of filing Mar 11, 2002
Gazette Date Apr 20, 2006
Gazette Date Apr 21, 2009
International application number JP2002002228
International publication number WO2002072152
Date of international filing Mar 11, 2002
Date of international publication Sep 19, 2002
Priority data
  • P2001-069655 (Mar 13, 2001) JP
  • P2001-130705 (Apr 27, 2001) JP
  • 2002WO-JP02228 (Mar 11, 2002) WO
Title Gene carriers with the use of polysaccharide and process for producing the same achieved
Abstract (US7521433)
Disclosed are gene carriers utilizing beta-1,3-glucan and methods of preparing the same.
The beta-1,3-glucan has at least one 1,6-glucopyranoside branch and is chemically modified through periodate oxidation and reductive amination so as to impart nucleic acid-binding functional groups (for example, cationic functional groups) to at least some of the 1,6-glucopyranoside branches thereof.
Triple helix beta-1,3-glucan is dissolved in a polar organic solvent to form single-stranded beta-1,3-glucan.
By replacing, in the presence of a nucleic acid, the polar organic solvent for the solution containing the chemically modified single-stranded beta-1,3-glucan by water, a complex (gene carrier) is formed in which the nucleic acid is bound to double-stranded beta-1,3-glucan.
Scope of claims [claim1]
1. A gene carrier composed of a complex in which a single-stranded nucleic acid is bound to a double-stranded beta -1,3-glucan wherein the beta -1,3-glucan has at least one 1,6-glucopyranoside branch per repeating unit of polysaccharide and is selected from schizophyllan, lentinan, grifolan, and scleroglucan, and wherein at least some of 1,6-glucopyranoside branches are chemically modified to be functionalized with nucleic acid-binding functional groups which are cationic functional groups formed by reaction of the polysaccharide with chain or cyclic compounds containing at least one primary or secondary amino group, wherein the chemical modification is carried out by periodate-oxidizing the 1,6-glucopyranoside branches and then reductive-aminating the periodated 1,6-glucopyranoside branches to impart the nucleic acid binding functional group thereto, and wherein the 1,6-glucopyranoside branch which has been functionalized with the nucleic acid-binding groups is expressed by the following general formula (1):
wherein the two X's are the cationic functional groups and are identical or different.
[claim2]
2. The gene carrier of claim 1, wherein the nucleic acid-binding functional groups are introduced at a rate of at least 0.1 mol % and up to 50 mole % based on the repeating unit of the polysaccharide.
[claim3]
3. The gene carrier of claim 1, wherein the beta -1,3-glucan has a molecular weight of at least 2000.
[claim4]
4. The gene carrier of claim 1, wherein the nucleic acid is an antisense DNA.
[claim5]
5. The gene carrier of claim 1, wherein the beta -1,3-glucan comprises schizophyllan.
  • Inventor, and Inventor/Applicant
  • KIMURA TARO
  • MIZU MASAMI
  • SAKURAI KAZUO
  • SHINKAI SEIJI
  • KOUMOTO KAZUYA
  • FUKUOKA PREFECTURAL GOVERNMENT
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • MITSUI SUGAR
IPC(International Patent Classification)
Reference ( R and D project ) PRESTO Organization and Function AREA
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