Top > Search of International Patents > Fluorescent sensor for phosphate ion and phosphorylated peptide

Fluorescent sensor for phosphate ion and phosphorylated peptide achieved

Foreign code F110005348
File No. K01205US
Posted date Aug 31, 2011
Country United States of America
Application number 50505603
Gazette No. 20050148086
Gazette No. 7521250
Date of filing Jan 27, 2003
Gazette Date Jul 7, 2005
Gazette Date Apr 21, 2009
International application number JP2003000705
International publication number WO2003071280
Date of international filing Jan 27, 2003
Date of international publication Aug 28, 2003
Priority data
  • P2002-045846 (Feb 22, 2002) JP
  • 2003WO-JP00705 (Jan 27, 2003) WO
Title Fluorescent sensor for phosphate ion and phosphorylated peptide achieved
Abstract (US7521250)
Disclosed is a fluorescent sensor for phosphate ion and phosphorylated peptide, which comprises a phosphate anion-selective fluorescent compound expressed by the following general formula (1).
In the formula (1), FL represents a fluorescent functional group or atomic group having an aromatic ring or heterocyclic ring (for example, dimethylanthlyl group), and X represents a functional group or atomic group which will be liberated in an aqueous solution to form an anion (for example, NO3):
Scope of claims [claim1]
1. A method comprising: in an aqueous solution, providing a fluorescent sensor for phosphate ion and phosphorylated peptide, which sensor comprises a phosphate anion-selective fluorescent compound expressed by the following general formula (1):
wherein FL represents a fluorescent functional group or atomic group having an aromatic ring or heterocyclic ring, and X represents a functional group or atomic group which is liberated in an aqueous solution to form an anion;
and
sensing at least one of phosphate ion and phosphorylated peptide in the aqueous solution as a change in fluorescent intensity, wherein said phosphorylated peptide is composed of an amino acid sequence containing a hydrophobic amino acid and an anionic amino acid in addition to a phosphorylated amino acid.
[claim2]
2. The method of claim 1, wherein FL is selected from one of (a), (b), (c) and (d) in the following (2):
wherein R represents hydrogen atom, an alkyl group having 1 to 4 carbon atoms or benzyl group in the formula (d).
[claim3]
3. The method of claim 1, wherein X is NO3, a halogen atom or ClO4.
[claim4]
4. The method of claim 2, wherein X is NO3, a halogen atom or ClO4.
[claim5]
5. The method of claim 1, wherein said phosphorylated peptide exhibits a change in fluorescence corresponding to a change in concentration of the phosphorylated peptide and said method includes determining a concentration of said phosphorylated peptide based on said change in fluorescence.
[claim6]
6. An aqueous solution comprising: a fluorescent sensor which comprises a phosphate anion-selective fluorescent compound expressed by the following general formula (1):
wherein FL represents a fluorescent functional group or atomic group having an aromatic ring or heterocyclic ring, and X represents a functional group or atomic group which is liberated in the aqueous solution to form an anion;
and
a phosphate ion or phosphorylated peptide in sufficient amount to produce a fluorescence change, wherein said phosphorylated peptide is composed of an amino acid sequence containing a hydrophobic amino acid and an anionic amino acid in addition to a phosphorylated amino acid.
[claim7]
7. The solution of claim 6, wherein FL is selected from one of (a), (b), (c) and (d) in the following (2):
wherein R represents hydrogen atom, an alkyl group having 1 to 4 carbon atoms or benzyl group in the formula (d).
[claim8]
8. The solution of claim 6, wherein X is NO3, a halogen atom or ClO4.
[claim9]
9. The solution of claim 7, wherein X is NO3, a halogen atom or ClO4.
[claim10]
10. A method comprising: in an aqueous solution containing a phosphorylated peptide, providing a fluorescent sensor which comprises a compound expressed by the following general formula (1):
wherein FL represents a fluorescent functional group or atomic group having an aromatic ring or heterocyclic ring, and X represents a functional group or atomic group which is liberated in an aqueous solution to form an anion;
and
sensing an exhibited change in fluorescent intensity of the solution corresponding to a change in concentration of the phosphorylated peptide in the aqueous solution.
  • Inventor, and Inventor/Applicant
  • HAMACHI ITARU
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
Reference ( R and D project ) PRESTO Organization and Function AREA
Please contact us by E-mail or facsimile if you have any interests on this patent.

PAGE TOP

close
close
close
close
close
close