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Method for measurement of concentration of antigen UPDATE

外国特許コード F110005399
整理番号 K02216WO
掲載日 2011年9月5日
出願国 アメリカ合衆国
出願番号 67100208
公報番号 20100248267
出願日 平成20年7月30日(2008.7.30)
公報発行日 平成22年9月30日(2010.9.30)
国際出願番号 JP2008002049
国際公開番号 WO2009016839
国際出願日 平成20年7月30日(2008.7.30)
国際公開日 平成21年2月5日(2009.2.5)
優先権データ
  • 特願2007-201147 (2007.8.1) JP
  • 2008WO-JP02049 (2008.7.30) WO
発明の名称 (英語) Method for measurement of concentration of antigen UPDATE
発明の概要(英語) (US20100248267)
The object of the present invention is to provide a detection method that can detect an antigen even if the target antigen has a low molecular weight in a simpler, more efficient, and more stable manner and with higher sensitivity.
The detection of the antigen is carried out by using a fusion protein comprising a polypeptide containing a VH domain of an antibody; a given protein; a linker peptide; a partner protein having an activity of binding to the protein and capable of detecting binding to the protein; and a polypeptide containing a VL domain of the antibody; in this order or in inverse order to this order, wherein the presence or absence of the binding of the polypeptide containing a VH domain and the polypeptide containing a VL domain to the antigen induces the presence or absence of the binding of the protein and the partner protein.
特許請求の範囲(英語) [claim1]
1. A fusion protein comprised of a polypeptide containing a VH domain of an antibody; a given protein; a linker peptide; a partner protein having a binding activity to the protein and capable of detecting the presence or absence of the binding to the protein; and a polypeptide containing a VL domain of the antibody; in this order or in reverse order, wherein the presence or absence of the binding of the polypeptide containing a VH domain and the polypeptide containing a VL domain to the antigen induces the presence or absence of the binding of the protein and the partner protein.
[claim2]
2. The fusion protein according to claim 1, wherein the combination of the given protein and the partner protein is a combination of a protein containing an N-terminal fragment of an enzyme protein and a protein containing a C-terminal fragment of the enzyme protein, and that the presence or absence of the binding of the protein containing the N-terminal fragment and the protein containing the C-terminal fragment can be detected by the change of the given enzyme activity of the enzyme protein.
[claim3]
3. The fusion protein according to claim 2, wherein the enzyme protein is beta -lactamase.
[claim4]
4. The fusion protein according to claim 3, wherein the protein containing an N-terminal fragment of beta -lactamase is a protein consisted of the amino acid sequence represented by SEQ ID NO: 5, and the protein containing a C-terminal fragment of beta -lactamase is a protein consisted of the amino acid sequence represented by SEQ ID NO: 7.
[claim5]
5. The fusion protein according to any one of claims 1 to 4, wherein the linker peptide is the amino acid sequence consisted of Asp-Lys-Ser.
[claim6]
6. The fusion protein according to any one of claims 1 to 5, wherein the sequence of a given protein; a linker peptide; and a partner protein is a protein consisted of the amino acid sequence represented by SEQ ID NO: 13.
[claim7]
7. A DNA encoding the fusion protein according to any one of claims 1 to 6.
[claim8]
8. A recombinant vector having the DNA according to claim 7.
[claim9]
9. A transformed cell transformed by the recombinant vector according to claim 8.
[claim10]
10. A method for detecting an antigen in a sample, comprising the steps of: contacting the sample with the fusion protein according to any one of claims 1 to 6 in which a polypeptide containing a VH domain and a polypeptide containing a VI, domain can bind to the antigen, or with the transformed cell according to claim 9; and detecting the binding of a partner protein and a given protein in the fusion protein or in a fusion protein in which the transformed cell is expressed.
[claim11]
11. A kit for detecting an antigen provided with the fusion protein according to any one of claims 1 to 6 which is a protein wherein a given enzyme activity exhibited by a given protein and/or a partner protein changes when the given protein and the partner protein are bound to each other compared to when they are not bound, or the transformed cell according to claim 9; and a chromogenic substrate or a fluorogenic substrate that can be detected due to the change of the enzyme activity.
  • 発明者/出願人(英語)
  • UEDA HIROSHI
  • KOJIMA MIKI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) PRESTO Structure Function and Measurement Analysis AREA
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