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Phase object identification device and method

Foreign code F110005401
File No. K02601WO
Posted date Sep 5, 2011
Country United States of America
Application number 92254209
Gazette No. 20110043816
Gazette No. 8891089
Date of filing Feb 27, 2009
Gazette Date Feb 24, 2011
Gazette Date Nov 18, 2014
International application number JP2009000879
International publication number WO2009119005
Date of international filing Feb 27, 2009
Date of international publication Oct 1, 2009
Priority data
  • P2008-080976 (Mar 26, 2008) JP
  • 2009WO-JP00879 (Feb 27, 2009) WO
Title Phase object identification device and method
Abstract (US8891089)
An object of the invention is to provide a phase object identification device and method which can identify a phase object in a completely different manner from conventional methods for observing or measuring a phase object.
A phase object identification device 1 for identifying a phase object for changing the phase of light includes a light source 2, a sample holding means 3 for holding a phase object 31 to be identified, a holographic recording medium 4 on which a hologram 41 formed by interference between reference light 25 and object light 24 that is phase-modulated by a known phase object 32 is recorded, and a light detector 5, a phase of light 21 emitted from the light source is modulated by the phase object to be identified to generate sample light 22, the hologram of the holographic recording medium is irradiated with the sample light, reproduced light 23 reproduced from the hologram of the holographic recording medium is detected by the light detector.
Scope of claims [claim1]
1. A phase object identification device for detecting a correlation between a phase object to be identified and a known phase object, the device comprising: a light source;
sample holding means for holding a phase object to be identified comprising sample positioning means for shifting the phase object to be identified in a planar direction orthogonal to an optical axis;
an observation optical system comprising a sample-side objective lens and an imaging lens or an eyepiece for observing an image of the phase object to be identified, the phase object held by the sample holding means, the observation optical system configured to form the image of the phase object to be identified;
focusing means for changing the distance between the phase object to be identified and the sample-side objective lens;
a holographic recording medium on which a hologram formed by interference between reference light and object light that is phase-modulated by a phase pattern of a known phase object is recorded;
a light detector; and
an identification optical system in which a phase of light emitted from the light source is modulated by a phase pattern of the phase object to be identified to generate sample light, the hologram of the holographic recording medium is irradiated with the sample light, and reproduced light reproduced from the hologram of the holographic recording medium is detected by the light detector, and
the sample-side objective lens of the observation optical system is also used as an optical component of the identification optical system,
wherein the observation optical system comprises a beam splitter configured to direct a portion of the generated sample light to an image sensor, and wherein the image of the phase object to be identified is formed by the image sensor utilizing said portion of the generated sample light that is excluded from passing through the hologram of the holographic recording medium.
[claim2]
2. The phase object identification device according to claim 1, characterized in that the hologram of the holographic recording medium is irradiated with the sample light by an objective lens arranged in such a way that a real image of the phase object to be identified is located on an incident pupil plane.
[claim3]
3. The phase object identification device according to claim 1, characterized in that multiple holograms formed from multiple known phase objects are recorded on the holographic recording medium, and the phase object identification device comprises irradiated position shifting means for shifting a position irradiated with the sample light in the holographic recording medium.
[claim4]
4. The phase object identification device according to claim 1, characterized in that it comprises sample conveying means for sequentially conveying multiple phase objects to be identified to the sample holding means.
[claim5]
5. The phase object identification device according to claim 1, characterized in that the phase object to be identified is a biological cell or a bacterium, and the presence or absence of a cell nucleus in the biological cell or the bacterium is identified.
[claim6]
6. The phase object identification device according to claim 1, characterized in that the known phase object is a specimen within standards, and whether or not the phase object to be identified corresponds to the standards is identified.
[claim7]
7. The phase object identification device according to claim 1, characterized in that it comprises reference light generation means for generating reference light, the sample holding means is able to hold a known phase object, a phase of light emitted from the light source is modulated by a phase pattern of the known phase object to generate object light, the reference light generation means generates reference light, the holographic recording medium is irradiated with the object light and the reference light, and a hologram formed by interference between the object light and the reference light is recorded on the holographic recording medium.
[claim8]
8. The phase object identification device according to claim 7, characterized in that the reference light generation means is an opening formed in the sample holding means.
[claim9]
9. A phase object identification method comprising the steps of: observing an image of a phase object to be identified utilizing an observation optical system;
adjusting a size of the observed image of the phase object to be identified by changing a distance between a sample-side objective lens of the observation optical system and the phase object to be identified;
adjusting a position of the observed image of the phase object to be identified by shifting the phase object to be identified in a planar direction orthogonal to an optical axis of the observation optical system, wherein, with the size and the position of the observed image of the phase object to be identified adjusted, a phase of light emitted from a light source is modulated by the phase pattern of the phase object to be identified to generate sample light;
irradiating with the generated sample light a holographic recording medium, on which a hologram formed by interference between reference light and object light that is phase-modulated by a phase pattern of a known phase object is recorded; and
detecting reproduced light reproduced from the hologram of the holographic recording medium by a light detector;
wherein the observation optical system comprises a beam splitter configured to direct a portion of the generated sample light to an image sensor, and wherein the image of the phase object to be identified is formed by the image sensor utilizing the portion of the generated sample light that is excluded from passing through the hologram of the holographic recording medium; and
wherein the phase object to be identified is identified as having a correlation with the known phase object if the intensity of the reproduced light detected by the light detector is greater than a threshold value, or the phase object to be identified is identified as having no correlation with the known phase object if the intensity of the reproduced light is less than the threshold value.
  • Inventor, and Inventor/Applicant
  • WATANABE ERIKO
  • KODATE KASHIKO
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
Reference ( R and D project ) PRESTO Life Phenomena and Measurement Analysis AREA
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