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Gene carrier achieved

Foreign code F110005411
File No. ITW-12-1US1
Posted date Sep 5, 2011
Country United States of America
Application number 46540203
Gazette No. 20030216346
Gazette No. 8017742
Date of filing Jun 19, 2003
Gazette Date Nov 20, 2003
Gazette Date Sep 13, 2011
Priority data
  • P1999-319470 (Nov 10, 1999) JP
  • P2000-142897 (May 16, 2000) JP
  • 2001US-09889117 (Sep 19, 2001) US
Title Gene carrier achieved
Abstract (US8017742)
Disclosed is a gene carrier composed of a hydrogen-bonding polymer having a polymer chain with hydrogen-bonding sites formed on the polymer chain, in which the polymer chain has a conformation similar to the conformation of a nucleic acid, and particularly, the polymer chain has helix parameters similar to the helix parameters of a nucleic acid of a helix structure.
In a preferred embodiment, the hydrogen-bonding polymer is composed of β-1,3-glucan such as schizophyllan.
The carrier can be expected to be applied as a vector and a separator for nucleic acids because it is capable of interacting with a nucleic acid to form a complex with the nucleic acid and thus carrying the nucleic acid.
Scope of claims [claim1]
1. An isolated nucleic acid-polymer complex composed of a bonded nucleic acid and a hydrogen bonding polymer having a polymer chain comprising a naturally occurring beta -1,3-glucan with hydrogen-bonding sites on the polymer chain, said hydrogen bonding sites being formed on said polymer chain by unbinding a beta -1,3-glucan to form a single stranded beta -1,3-glucan capable of forming a complex with a single stranded nucleic acid, said single stranded nucleic acid comprising a poly(dA), poly(A), or poly(C) nucleic acid and, when being combined with the single stranded beta -1,3-glucan, said poly(dA), poly(A), or poly(C) nucleic acid being a helical compound, and said polymer chain, when in aqueous solution, having helix parameters similar to the helix parameters of the poly(dA), poly(A), or poly(C) of the single stranded nucleic acid, the similar helix parameters including an h/R value in a range of 2 to 4 .ANG., wherein the bonded nucleic acid is bonded to the hydrogen bonding polymer through the hydrogen-bonding sites.
[claim2]
2. The nucleic acid polymer complex of claim 1, wherein the naturally occurring beta -1,3-glucan is schizophyllan.
[claim3]
3. The nucleic acid polymer complex of claim 1, wherein the bonded nucleic acid comprises RNA or DNA.
[claim4]
4. The nucleic acid polymer complex of claim 1, wherein the beta -1,3-glucan is curdlan, lentinan, pachyman, griffollan or sceroglucan.
[claim5]
5. The nucleic acid polymer complex of claim 1, wherein the hydrogen-bonding polymer has a weight-average molecular weight of 2000 or more.
[claim6]
6. The nucleic acid polymer complex of claim 4, wherein the wherein the hydrogen-bonding polymer has a weight-average molecular weight of 6000 or more.
[claim7]
7. An aqueous solution comprising the nucleic acid-polymer complex of claim 1.
[claim8]
8. A method of forming an isolated nucleic acid-polymer complex composed of a bonded nucleic acid and a hydrogen bonding polymer having a polymer chain comprising a beta -1,3-glucan with hydrogen-bonding sites on the polymer chain, comprising: unbinding a triple helix structure comprising a naturally occurring beta -1,3-glucan to form a single stranded beta -1,3-glucan comprising a hydrogen bonding polymer having a polymer chain with hydrogen-bonding sites formed on the polymer chain; and
combining the hydrogen bonding polymer with an aqueous solution of a single stranded nucleic acid comprising poly(dA), poly(A), or poly(C) to form the complex, said polymer chain, when in said aqueous solution, having helix parameters similar to the helix parameters of the poly(dA), poly(A), or poly(C) of the single stranded nucleic acid, wherein said poly(dA), poly(A), or poly(C) of the single stranded nucleic acid and the hydrogen bonding polymer are helical compounds, the similar helix parameters including an h/R value in a range of 2 to 4 .ANG., whereby the bonded nucleic acid is bonded to the hydrogen bonding polymer through the hydrogen-bonding sites.
[claim9]
9. The method of claim 8, wherein the beta -1,3-glucan is schizophyllan.
[claim10]
10. An isolated nucleic acid-polymer complex composed of a nucleic acid and a hydrogen bonding polymer having a polymer chain with hydrogen-bonding sites on the polymer chain, wherein the nucleic acid is bonded to the hydrogen bonding polymer through the hydrogen-bonding sites, said polymer chain having a twining direction which is the same as that of the nucleic acid, the hydrogen bonding polymer being partially or wholly composed of a naturally occurring beta -1,3-glucan and the nucleic acid being bonded comprising a single-stranded nucleic acid comprising poly(dA), poly(A), or poly(C) residues, the poly(dA), poly(A), or poly(C) residues forming a helical compound.
[claim11]
11. The nucleic acid polymer complex of claim 10, wherein the beta -1,3-glucan is schizophyllan.
[claim12]
12. The nucleic acid polymer complex of claim 10, wherein the nucleic acid comprises RNA or DNA.
[claim13]
13. The nucleic acid polymer complex of claim 10, wherein the beta -1,3-glucan is curdlan, lentinan, pachyman, griffollan or scleroglucan.
[claim14]
14. The nucleic acid polymer complex of claim 10, wherein the hydrogen-bonding polymer has a weight-average molecular weight of 2000 or more.
[claim15]
15. The nucleic acid polymer complex of claim 10, wherein the hydrogen-bonding polymer has an h/R value in a range of 2 to 4 .ANG..
[claim16]
16. An aqueous solution comprising the nucleic acid-polymer complex of claim 10.
[claim17]
17. An isolated nucleic acid-polymer complex composed of a nucleic acid and a hydrogen bonding polymer having a polymer chain, the hydrogen bonding polymer consisting of beta -1,3-glucan, the complex being formed by a method comprising: unbinding a triple helix structure comprising a naturally occurring beta -1,3-glucan to form a single strand comprising a hydrogen bonding polymer having a polymer chain with hydrogen-bonding sites on the polymer chain; and
combining the hydrogen bonding polymer with an aqueous solution of a single stranded nucleic acid to form the complex through the hydrogen-bonding sites on the polymer chain, the single stranded nucleic acid comprising poly(A), poly (C) or poly(dA), the poly(A), poly (C) or poly(dA) of the nucleic acid being a helical compound which has helix parameters similar to that of the hydrogen bonding polymer.
[claim18]
18. The nucleic acid polymer complex of claim 17, wherein the polymer chain has an h/R value in a range of 2 to 4 .ANG..
[claim19]
19. The isolated nucleic acid-polymer complex of claim 17, wherein the unbinding of the triple helix structure includes dissolving the beta -1,3-glucan in a solvent.
[claim20]
20. The isolated nucleic acid-polymer complex of claim 19, wherein the solvent comprises DMSO.
[claim21]
21. The isolated nucleic acid-polymer complex of claim 17, wherein the nucleic acid is an antisense DNA linked to a poly(dA).
[claim22]
22. The isolated nucleic acid-polymer complex of claim 17, wherein said polymer chain has helix parameters sufficiently similar to the helix parameters of the nucleic acid for a complex to be formed between said nucleic acid and said hydrogen bonding polymer.
[claim23]
23. The nucleic acid polymer complex of claim 17, wherein the naturally occurring beta -1,3-glucan is schizophyllan.
  • Inventor, and Inventor/Applicant
  • SAKURAI KAZUO
  • SHINKAI SEIJI
  • KIMURA TARO
  • TABATA KENGO
  • KOUMOTO KAZUYA
  • GRONWALD OLIVER
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • MITSUI SUGAR
  • SHIN MITSUI SUGAR
IPC(International Patent Classification)
U.S. Cl./(Sub)
  • A61K047/48K8
  • A61K047/48R2T
  • A61K048/00
  • C07H015/04
  • C07H021/00
  • C08B037/00M3
  • C12N015/87
  • M07B200/11
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