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MUTANT FOR INCREASING OLEIC ACID CONTENT OF SOYBEAN OIL AND FAT, AND RESPONSIBLE GENE THEREFOR

外国特許コード F110005415
整理番号 S2009-0506-C0
掲載日 2011年9月6日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2010JP060928
国際公開番号 WO 2010150901
国際出願日 平成22年6月21日(2010.6.21)
国際公開日 平成22年12月29日(2010.12.29)
優先権データ
  • 特願2009-147706 (2009.6.22) JP
発明の名称 (英語) MUTANT FOR INCREASING OLEIC ACID CONTENT OF SOYBEAN OIL AND FAT, AND RESPONSIBLE GENE THEREFOR
発明の概要(英語) A soybean having an oleic acid content exceeding that of the high-oleic-acid soybeans developed thus far is provided without using gene recombination. A soybean species containing a mutant gene in which the function of the GmFAD2-1b gene has been deleted, and a mutant gene in which the function of the GmFAD2-1b gene has been deleted are also provided.
従来技術、競合技術の概要(英語) BACKGROUND ART
Soybean, is produced in high world source vegetable fats. The composition of the fatty acids contained in vegetable fats, for which directly affect the quality of the fats and oils, the modification of fatty acid composition in soybean breeding major has been a problem. In general, in fatty acid composition, a high content of saturated fatty acids is stable and the quality of the fats and oils, or fats in this way ingest low-density lipoprotein cholesterol blood level is raised, not preferable in terms of nutritional. Also, such as a polyunsaturated fatty acid is linoleic acid, is easily oxidized and unstable and, when it is used as the bio-sludge is likely to occur. Conventionally, instability of the polyunsaturated fatty acid as a means for compensating the hydrogen addition are performed, for cost reasons and is not economical, in addition, as a by-product considered risk factors for coronary disease resulting in the trans-fatty acid is not preferable. In contrast, monounsaturated fatty acids such as oleic acid, relatively stable against oxidation, to reduce the cholesterol level in blood from, soybean contains a large amount of oleic acid, also as a fuel source, it is also preferred as a source of nutrient. Generally flows in the composition of the fat obtained from the seeds of soybean, the amount of oleic acid is about 20-25%, since the amount of 50-57% linoleic acid, oleic acid content is increased so as to reduce the number of breeding have been made. However, varieties developed to date, practically sufficient oleic acid content not been obtained. By previous studies, an oleic acid content of vegetable fats in order to increase, oleic acid and linoleic acid desaturase produced during the endoplasmic reticulum-type omega -6 catalyzes fatty acid desaturase (FAD2) it is important to reduce the activity of the clear (non-patent document 1) and the, FAD2 gene encoding this enzyme at the time of the target for breeding. FAD2 Using gene recombinant technology as genetically modified soybean, cosuppression method, inhibits expression of the gene FAD2 ultra high oleic acid soybean (systematic name: 260-05) and have been developed, an oleic acid content was increased to about 80% or more are reported (patent document 1 and non-patent document 2). In addition, recombinantly, the ratio of saturated and unsaturated fatty acids involved in the regulation of the expression of the gene encoding palmitoyl - ACP thioesterase by reducing, increased oleic acid content have been reported example (patent document 2). However, these high oleic acid soybean it was found that the recombinant gene, finally unacceptable to the consumer, it is presently not production for human consumption. Therefore, high-oleic acid-containing edible soybean or soybean oil provided for the purpose of the case, without using gene recombinant technology to provide high oleic soybean is desired. Previously, the present inventors have mutations of the FAD2 gene by site-directed mutagenesis which is one of the functions of the GmFAD2-1a 2 gene mutations abolished (M23 and KK21) was prepared in the system, from about 25% oleic acid content of the composition to 50% or more success to doubled (patent document 3 and non-patent document 1), without using gene recombinant technology by further increasing the oleic acid content is difficult.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • SAGA UNIVERSITY
  • 発明者(英語)
  • ANAI, Toyoaki
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT RO RS RU SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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