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Method of amplifying ATP and use thereof 新技術説明会 実績あり

外国特許コード F110005469
整理番号 K05323US2
掲載日 2011年9月6日
出願国 アメリカ合衆国
出願番号 76910010
公報番号 20100311093
公報番号 8003341
出願日 平成22年4月28日(2010.4.28)
公報発行日 平成22年12月9日(2010.12.9)
公報発行日 平成23年8月23日(2011.8.23)
国際出願番号 JP2004011186
国際公開番号 WO2005010208
国際出願日 平成16年7月27日(2004.7.27)
国際公開日 平成17年2月3日(2005.2.3)
優先権データ
  • 特願2003-202992 (2003.7.29) JP
  • 2004US-10566957 (2004.7.27) US
  • 2004WO-JP11186 (2004.7.27) WO
発明の名称 (英語) Method of amplifying ATP and use thereof 新技術説明会 実績あり
発明の概要(英語) (US8003341)
The ATP amplification method is a method for amplifying and detecting a very trace amount of exogenous ATP by allowing a fusion protein (PPK-ADK) of a polyphosphate kinase and an adenylate kinase, the fusion protein not containing ADP, to act on a mixture of ATP, AMP, and a polyphosphate compound.
Also provided is an ultrasensitive ATP amplification method by which ATP at a single cell level can be amplified and detected, and an ultrasensitive microbial assay based on this ATP amplification method.
特許請求の範囲(英語) [claim1]
1. A kit for detecting the presence of a microorganism, comprising an adenosine triphosphate amplification reagent containing adenosine monophosphate, a polyphosphate compound, and a fusion protein that has a polyphosphate kinase and an adenylate kinase in this order from the N-terminal; and an adenosine triphosphate detection reagent for detecting adenosine triphosphate, wherein the fusion protein has been subjected to an apyrase treatment and a pyrophosphate treatment so as to remove adenosine diphosphate bound to the fusion protein and wherein the fusion protein is not present in a complex with adenosine diphosphate.
[claim2]
2. The kit of claim 1, further comprising a cell lysis reagent.
[claim3]
3. A fusion protein that has a polyphosphate kinase and an adenylate kinase in this order from the N-terminal and that has been subjected to an apyrase treatment and a pyrophosphate treatment so as to remove adenosine diphosphate bound to the fusion protein, wherein the fusion protein is not present in a complex with adenosine diphosphate.
  • 発明者/出願人(英語)
  • KURODA AKIO
  • ENROOT PRODUCTS
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • C12N009/12F
  • C12N015/62
  • C12P019/32
  • C12Q001/48B
参考情報 (研究プロジェクト等) PRESTO Conversion and Control by Advanced Chemistry AREA
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