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Method of amplifying ATP and use thereof meetings achieved

Foreign code F110005469
File No. K05323US2
Posted date Sep 6, 2011
Country United States of America
Application number 76910010
Gazette No. 20100311093
Gazette No. 8003341
Date of filing Apr 28, 2010
Gazette Date Dec 9, 2010
Gazette Date Aug 23, 2011
International application number JP2004011186
International publication number WO2005010208
Date of international filing Jul 27, 2004
Date of international publication Feb 3, 2005
Priority data
  • P2003-202992 (Jul 29, 2003) JP
  • 2004US-10566957 (Jul 27, 2004) US
  • 2004WO-JP11186 (Jul 27, 2004) WO
Title Method of amplifying ATP and use thereof meetings achieved
Abstract (US8003341)
The ATP amplification method is a method for amplifying and detecting a very trace amount of exogenous ATP by allowing a fusion protein (PPK-ADK) of a polyphosphate kinase and an adenylate kinase, the fusion protein not containing ADP, to act on a mixture of ATP, AMP, and a polyphosphate compound.
Also provided is an ultrasensitive ATP amplification method by which ATP at a single cell level can be amplified and detected, and an ultrasensitive microbial assay based on this ATP amplification method.
Scope of claims [claim1]
1. A kit for detecting the presence of a microorganism, comprising an adenosine triphosphate amplification reagent containing adenosine monophosphate, a polyphosphate compound, and a fusion protein that has a polyphosphate kinase and an adenylate kinase in this order from the N-terminal; and an adenosine triphosphate detection reagent for detecting adenosine triphosphate, wherein the fusion protein has been subjected to an apyrase treatment and a pyrophosphate treatment so as to remove adenosine diphosphate bound to the fusion protein and wherein the fusion protein is not present in a complex with adenosine diphosphate.
[claim2]
2. The kit of claim 1, further comprising a cell lysis reagent.
[claim3]
3. A fusion protein that has a polyphosphate kinase and an adenylate kinase in this order from the N-terminal and that has been subjected to an apyrase treatment and a pyrophosphate treatment so as to remove adenosine diphosphate bound to the fusion protein, wherein the fusion protein is not present in a complex with adenosine diphosphate.
  • Inventor, and Inventor/Applicant
  • KURODA AKIO
  • ENROOT PRODUCTS
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
Reference ( R and D project ) PRESTO Conversion and Control by Advanced Chemistry AREA
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