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Hollow nanoparticle of NBsAg large protein for drug delivery 実績あり

外国特許コード F110005560
整理番号 P023P03US
掲載日 2011年9月8日
出願国 アメリカ合衆国
出願番号 50924903
公報番号 20060088536
公報番号 7951379
出願日 平成15年3月26日(2003.3.26)
公報発行日 平成18年4月27日(2006.4.27)
公報発行日 平成23年5月31日(2011.5.31)
国際出願番号 JP2003003694
国際公開番号 WO2003082330
国際出願日 平成15年3月26日(2003.3.26)
国際公開日 平成15年10月9日(2003.10.9)
優先権データ
  • 特願2002-097424 (2002.3.29) JP
  • 特願2003-045088 (2003.2.21) JP
  • 2003WO-JP03694 (2003.3.26) WO
発明の名称 (英語) Hollow nanoparticle of NBsAg large protein for drug delivery 実績あり
発明の概要(英語) (US7951379)
The invention provides a therapeutic drug that uses hollow protein nanoparticles displaying an antibody against a specific cell or specific tissue.
The effectiveness of the drug has been proved by animal testing.
The invention also provides a therapeutic method using such a drug.
In a drug according to the present invention, a substance to be transferred into a cell for treating a disease (for example, a cancer treating gene such as a thymidine kinase gene derived from simple herpes virus) is encapsulated in hollow nanoparticles of a particle-forming protein (for example, hepatitis B virus surface-antigen protein that has been modified to lack its infectivity to hepatocytes and display an antibody).
The particle surface of the drug displays an antibody, such as a cancer specific antibody, that recognizes an antigen molecule displayed on the surface of a specific cancer cell.
特許請求の範囲(英語) [claim1]
1. A hollow nanoparticle for delivering a substance to a cell, comprising a modified HBV surface antigen large (HBsAg L) protein formed into a particle, an antibody displayed on the surface of the particle, and a substance encapsulated inside the particle, wherein the modified HBsAg L protein includes a replacement of amino acids 50 to 153 in a pre-S region of the HBsAg L protein, corresponding to HBV serotype y, by a ZZ tag consisting of SEQ ID NO: 29, and wherein the antibody binds to the ZZ tag fused to the HBsAg L protein.
[claim2]
2. The hollow nanoparticle of claim 1, wherein the antibody is a single chain antibody.
[claim3]
3. The hollow nanoparticle of claim 1, wherein the modified HBsAg L protein is expressed in a eukaryotic cell.
[claim4]
4. The hollow nanoparticle of claim 3, wherein the eukaryotic cell is selected from a group consisting of a yeast cell, an insect cell, and an animal cell.
[claim5]
5. The hollow nanoparticle of claim 1, wherein the substance comprises a gene.
[claim6]
6. The hollow nanoparticle of claim 5, wherein the gene comprises a gene of thymidine kinase derived from simplex herpes virus.
[claim7]
7. A hollow nanoparticle comprising a modified HBV surface antigen large (HBsAg L) protein formed into a particle, wherein the modified HBsAg L protein includes a replacement of amino acids 50 to 153 in a pre-S region of the HBsAg L protein, corresponding to HBV serotype y, by a ZZ tag consisting of SEQ ID NO: 29.
  • 発明者/出願人(英語)
  • KURODA SHUNICHI
  • TANIZAWA KATSUYUKI
  • OKAJIMA TOSHIHIDE
  • KONDO AKIHIKO
  • UEDA MASAKAZU
  • SENO MASAHARU
  • BEACLE VIB - VLAAMS INSTITUUT VOOR BIOTECHNOLOGIE
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • A61K009/51H8B
  • A61K047/48T4B28
  • A61K047/48W8D
  • A61K048/00B4B
  • B82Y005/00
  • C07K014/005
  • C07K016/18
  • C07K016/28G
  • C07K016/40
  • C12N007/00
  • C12N015/88
  • K61K039/555B5
  • M07K317/622
  • M12N730/10122
  • M12N730/10123
  • M12N810/85G
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