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Biocompatible transparent sheet, method for producing the same, and cultured cell sheet used the same sheet

Foreign code F110005589
File No. RJ008P96WO
Posted date Sep 8, 2011
Country United States of America
Application number 22525607
Gazette No. 20090311297
Gazette No. 8454987
Date of filing Mar 14, 2007
Gazette Date Dec 17, 2009
Gazette Date Jun 4, 2013
International application number JP2007055028
International publication number WO2007108373
Date of international filing Mar 14, 2007
Date of international publication Sep 27, 2007
Priority data
  • P2006-074254 (Mar 17, 2006) JP
  • 2007WO-JP55028 (Mar 14, 2007) WO
Title Biocompatible transparent sheet, method for producing the same, and cultured cell sheet used the same sheet
Abstract (US8454987)
The present invention provides a flexible, biocompatible transparent sheet (8) which has high biocompatibility and a high ability to adsorb a biologically relevant substance, which can be used as a novel biomaterial, and which can be used to observe the propagation, differentiation, and/or the like of living cells in real time.
The biocompatible transparent sheet (8) is produced in such a manner that a biocompatible ceramic film (2) is formed on a substrate (1) soluble in a solvent (11) incapable of dissolving biocompatible ceramics by using exemplary a laser ablation process, the substrate (1) depositing the film (2) is dipped in the solvent (11) and dissolved off, and the isolated film (2) is then dried.
The biocompatible transparent sheet (8) is utilizable of producing a flexible cultured cell sheet which can be directly transplanted to an affected area.
The cell sheet can be prepared in such a manner that cells are seeded on the biocompatible transparent sheet (8) and then propagated.
Scope of claims [claim1]
1. A method for producing a biocompatible transparent sheet, comprising: forming a biocompatible ceramic film on a substrate, the substrate being made of a water-soluble inorganic salt; and
dissolving the substrate having the biocompatible ceramic film by dipping the substrate in a polar solvent.
[claim2]
2. The biocompatible transparent sheet-producing method according to claim 1, wherein the biocompatible ceramic film is formed by any one of a laser ablation process, a sputtering process, an ion beam deposition process, an electron beam deposition process, a vacuum evaporation process, a molecular beam epitaxy process, and a chemical vapor deposition process.
[claim3]
3. The biocompatible transparent sheet-producing method according to claim 1, further comprising: drying the biocompatible ceramic film after said dissolving of the substrate.
[claim4]
4. The biocompatible transparent sheet-producing method according to claim 1, further comprising: heat-treating the biocompatible ceramic film in a high-temperature atmosphere containing at least one of a steam-containing gas and a carbon dioxide-containing gas, said heat-treating being performed after said forming of the biocompatible ceramic film.
[claim5]
5. The biocompatible transparent sheet-producing method according to claim 1, wherein the biocompatible ceramic film is made of hydroxyapatite.
[claim6]
6. The biocompatible transparent sheet-producing method according to claim 1, wherein the biocompatible ceramic film comprises different types of biocompatible ceramics.
[claim7]
7. The biocompatible transparent sheet-producing method according to claim 3, further comprising: heat-treating the biocompatible ceramic film in a high-temperature atmosphere containing at least one of a steam-containing gas and a carbon dioxide-containing gas, said heat-treating being performed after said forming of the biocompatible ceramic film or after said drying of the biocompatible ceramic film.
[claim8]
8. A method for producing a biocompatible transparent sheet, comprising: forming a biocompatible ceramic film on a substrate, the substrate including a portion which is made of a water-soluble inorganic salt, the substrate having a projection; and
dissolving the soluble portion of the substrate having the biocompatible ceramic film by dipping the substrate in a polar solvent.
[claim9]
9. The biocompatible transparent sheet-producing method according to claim 8, wherein the substrate includes a portion that is insoluble in the polar solvent in addition to the polar solvent-soluble portion.
[claim10]
10. The biocompatible transparent sheet-producing method according to claim 1, wherein the water-soluble inorganic salt is selected from the group consisting of sodium chloride, potassium chloride, an alkali metal halide, amorphous magnesium oxide, and a combination thereof.
[claim11]
11. The biocompatible transparent sheet-producing method according to claim 1, wherein the polar solvent is aqueous solvent.
[claim12]
12. The biocompatible transparent sheet-producing method according to claim 1, wherein the polar solvent is selected from the group consisting of pure water, a buffer solution for cell culture, a liquid culture medium for cell culture, and a combination thereof.
[claim13]
13. The biocompatible transparent sheet-producing method according to claim 1, wherein the biocompatible transparent sheet has a thickness of 4 to 50 mu m.
[claim14]
14. The biocompatible transparent sheet-producing method according to claim 8, wherein the water-soluble inorganic salt is selected from the group consisting of sodium chloride, potassium chloride, an alkali metal halide, amorphous magnesium oxide, and a combination thereof.
[claim15]
15. The biocompatible transparent sheet-producing method according to claim 8, wherein the polar solvent is aqueous solvent.
[claim16]
16. The biocompatible transparent sheet-producing method according to claim 8, wherein the polar solvent is selected from the group consisting of pure water, a buffer solution for cell culture, a liquid culture medium for cell culture, and a combination thereof.
[claim17]
17. The biocompatible transparent sheet-producing method according to claim 8, wherein the biocompatible transparent sheet has a thickness of 4 to 50 mu m.
  • Inventor, and Inventor/Applicant
  • HONTSU SHIGEKI
  • NISHIKAWA HIROAKI
  • KUSUNOKI MASANOBU
  • HATANAKA RYOUTA
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • KINKI UNIVERSITY
IPC(International Patent Classification)
U.S. Cl./(Sub)
  • A61L027/32
  • A61L027/38
  • C01B025/32
  • C12M023/12
  • C12M023/20
  • C12M023/22
  • C12M023/26
  • C12M025/06
  • C12N005/00S
  • C23C014/00B
  • C23C014/08D
  • C23C014/08F
  • C23C014/28
  • M12N533/14
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