Sugar-immobilized metal nanoparticle, method for measuring sugar-protein interaction using the same and method for recovering protein from sugar-protein interactant
外国特許コード | F110005671 |
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整理番号 | V350P001WO |
掲載日 | 2011年9月9日 |
出願国 | アメリカ合衆国 |
出願番号 | 92087806 |
公報番号 | 20090240032 |
公報番号 | 8067393 |
出願日 | 平成18年5月26日(2006.5.26) |
公報発行日 | 平成21年9月24日(2009.9.24) |
公報発行日 | 平成23年11月29日(2011.11.29) |
国際出願番号 | JP2006310592 |
国際公開番号 | WO2006126689 |
国際出願日 | 平成18年5月26日(2006.5.26) |
国際公開日 | 平成18年11月30日(2006.11.30) |
優先権データ |
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発明の名称 (英語) |
Sugar-immobilized metal nanoparticle, method for measuring sugar-protein interaction using the same and method for recovering protein from sugar-protein interactant
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発明の概要(英語) |
(US8067393) It is intended to provide a stable novel sugar-immobilized metal nanoparticle capable of easily immobilizing a sugar chain, a method for measuring sugar-protein interaction easily and at a low cost using the same without labeling, and a method for simply recovering a protein from a sugar-protein interactant. A maltose-immobilized gold nanoparticle was obtained by binding a ligand complex, in which maltose and a linker compound had been bound to each other, to a gold nanoparticle. By adding this maltose-immobilized gold nanoparticle to a dilution series of concanavalin A, a sugar-protein interactant of maltose and ConA was formed, and red-purple color derived from a colloidal solution of maltose-immobilized gold nanoparticle disappeared. That is, sugar-protein interaction could be confirmed by visual observation without labeling. |
特許請求の範囲(英語) |
[claim1] 1. A sugar-immobilized metal nanoparticle, which comprises: a ligand complex of a structure including a linker compound comprising thioctic acid and m-phenylenediamine at a molar ratio of 1:1, and a sugar having a reducing end, the linker compound including a hydrocarbon structure which includes: a hydrocarbon inducing chain with an aromatic amino group at an end and a carbon-nitrogen bond in a backbone; and a sulfur atom, the sugar being bound to the linker compound via the amino group; and gold bound to the ligand complex, wherein the sugar-immobilized metal nanoparticle is obtained by mixing the ligand complex with a solution of a gold nanoparticle prepared by mixing sodium tetrachloroaurate (III) with sodium borohydride, wherein the sugar-immobilized metal nanoparticle is stable, wherein a solution of the sugar-immobilized metal nanoparticle contains sodium tetrachloroaurate (III) at a concentration of 0.5 mM to 4 mM, sodium borohydride at a concentration that is 3 to 10 times a molar concentration of gold ion, and the ligand complex at a concentration of 10 mu M to 1000 mu M, and wherein the sugar-immobilized metal nanoparticle forms a sugar-protein interactant through interaction of a sugar and a protein, and wherein the sugar-immobilized metal nanoparticle forms a colloid solution when mixed with an aqueous solution. [claim2] 2. A method for measuring sugar-protein interaction, comprising the step of forming a sugar-protein interactant through interaction of sugar and protein by mixing a solution containing the sugar-immobilized metal nanoparticle of claim 1 with a protein that recognizes the sugar situated at the end of the sugar-immobilized metal nanoparticle. [claim3] 3. A method for recovering a protein from a sugar-protein interactant, comprising the steps of: forming a sugar-protein interactant through interaction of sugar and protein by mixing a solution containing the sugar-immobilized metal nanoparticle of claim 1 with a protein that recognizes the sugar situated at the end of the sugar-immobilized metal nanoparticle; and adjusting a pH of an admixture solution of the sugar-protein interactant and water to 5 or less. [claim4] 4. A method for recovering a protein from a sugar-protein interactant, comprising the steps of: forming a sugar-protein interactant through interaction of sugar and protein by mixing a solution containing the sugar-immobilized metal nanoparticle of claim 1 with a protein that recognizes the sugar situated at the end of the sugar-immobilized metal nanoparticle; and mixing the sugar-protein interactant with a sugar that is recognizable by the protein. |
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米国特許分類/主・副 |
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日本語項目の表示
発明の名称 |
新規糖固定化金属ナノ粒子およびこれを用いて糖-タンパク質相互作用を測定する方法、並びに糖-タンパク質相互作用体からタンパク質を回収する方法
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