Sugar-immobilized metal nanoparticle, method for measuring sugar-protein interaction using the same and method for recovering protein from sugar-protein interactant
| 外国特許コード | F110005671 |
|---|---|
| 整理番号 | V350P001WO |
| 掲載日 | 2011年9月9日 |
| 出願国 | アメリカ合衆国 |
| 出願番号 | 92087806 |
| 公報番号 | 20090240032 |
| 公報番号 | 8067393 |
| 出願日 | 平成18年5月26日(2006.5.26) |
| 公報発行日 | 平成21年9月24日(2009.9.24) |
| 公報発行日 | 平成23年11月29日(2011.11.29) |
| 国際出願番号 | JP2006310592 |
| 国際公開番号 | WO2006126689 |
| 国際出願日 | 平成18年5月26日(2006.5.26) |
| 国際公開日 | 平成18年11月30日(2006.11.30) |
| 優先権データ |
|
| 発明の名称 （英語） |
Sugar-immobilized metal nanoparticle, method for measuring sugar-protein interaction using the same and method for recovering protein from sugar-protein interactant
|
| 発明の概要（英語） |
(US8067393) It is intended to provide a stable novel sugar-immobilized metal nanoparticle capable of easily immobilizing a sugar chain, a method for measuring sugar-protein interaction easily and at a low cost using the same without labeling, and a method for simply recovering a protein from a sugar-protein interactant. A maltose-immobilized gold nanoparticle was obtained by binding a ligand complex, in which maltose and a linker compound had been bound to each other, to a gold nanoparticle. By adding this maltose-immobilized gold nanoparticle to a dilution series of concanavalin A, a sugar-protein interactant of maltose and ConA was formed, and red-purple color derived from a colloidal solution of maltose-immobilized gold nanoparticle disappeared. That is, sugar-protein interaction could be confirmed by visual observation without labeling. |
| 特許請求の範囲（英語） |
[claim1] 1. A sugar-immobilized metal nanoparticle, which comprises: a ligand complex of a structure including a linker compound comprising thioctic acid and m-phenylenediamine at a molar ratio of 1:1, and a sugar having a reducing end, the linker compound including a hydrocarbon structure which includes: a hydrocarbon inducing chain with an aromatic amino group at an end and a carbon-nitrogen bond in a backbone; and a sulfur atom, the sugar being bound to the linker compound via the amino group; and gold bound to the ligand complex, wherein the sugar-immobilized metal nanoparticle is obtained by mixing the ligand complex with a solution of a gold nanoparticle prepared by mixing sodium tetrachloroaurate (III) with sodium borohydride, wherein the sugar-immobilized metal nanoparticle is stable, wherein a solution of the sugar-immobilized metal nanoparticle contains sodium tetrachloroaurate (III) at a concentration of 0.5 mM to 4 mM, sodium borohydride at a concentration that is 3 to 10 times a molar concentration of gold ion, and the ligand complex at a concentration of 10 mu M to 1000 mu M, and wherein the sugar-immobilized metal nanoparticle forms a sugar-protein interactant through interaction of a sugar and a protein, and wherein the sugar-immobilized metal nanoparticle forms a colloid solution when mixed with an aqueous solution. [claim2] 2. A method for measuring sugar-protein interaction, comprising the step of forming a sugar-protein interactant through interaction of sugar and protein by mixing a solution containing the sugar-immobilized metal nanoparticle of claim 1 with a protein that recognizes the sugar situated at the end of the sugar-immobilized metal nanoparticle. [claim3] 3. A method for recovering a protein from a sugar-protein interactant, comprising the steps of: forming a sugar-protein interactant through interaction of sugar and protein by mixing a solution containing the sugar-immobilized metal nanoparticle of claim 1 with a protein that recognizes the sugar situated at the end of the sugar-immobilized metal nanoparticle; and adjusting a pH of an admixture solution of the sugar-protein interactant and water to 5 or less. [claim4] 4. A method for recovering a protein from a sugar-protein interactant, comprising the steps of: forming a sugar-protein interactant through interaction of sugar and protein by mixing a solution containing the sugar-immobilized metal nanoparticle of claim 1 with a protein that recognizes the sugar situated at the end of the sugar-immobilized metal nanoparticle; and mixing the sugar-protein interactant with a sugar that is recognizable by the protein. |
|
|
| 国際特許分類(IPC) |
|
| 米国特許分類/主・副 |
|
日本語項目の表示
| 発明の名称 |
新規糖固定化金属ナノ粒子およびこれを用いて糖－タンパク質相互作用を測定する方法、並びに糖－タンパク質相互作用体からタンパク質を回収する方法
|
|---|
※
ライセンスをご希望の方、特許の内容に興味を持たれた方は、問合せボタンを押してください。
『 Sugar-immobilized metal nanoparticle, method for measuring sugar-protein interaction using the same and method for recovering protein from sugar-protein interactant 』に関するお問合せ
- 国立研究開発法人科学技術振興機構（ＪＳＴ） 知的財産マネジメント推進部
- URL: http://www.jst.go.jp/chizai/
-
E-mail:
- Address: 〒102-8666 東京都千代田区四番町5-3
- TEL: 03-5214-8293
- FAX: 03-5214-8476
※ 同じキーワードでJ-STORE内を検索することが出来ます。→ J-STORE内を検索
