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Transposase and method of gene modification achieved

Foreign code F110005675
File No. Y0013US
Posted date Sep 9, 2011
Country United States of America
Application number 14863900
Gazette No. 6094853
Gazette No. 7034115
Date of filing Nov 14, 2000
Gazette Date Aug 1, 2000
Gazette Date Apr 25, 2006
International application number JP2000008014
International publication number WO2001040477
Date of international filing Nov 14, 2000
Date of international publication Jun 7, 2001
Priority data
  • P1999-345508 (Dec 3, 1999) JP
  • P2000-109033 (Apr 11, 2000) JP
  • 2000WO-JP08014 (Nov 14, 2000) WO
Title Transposase and method of gene modification achieved
Abstract (US7034115)
A transposase encoded by the Tol2 element; a polynucleotide encoding the same; a method of modifying the gene structure of a cell (preferably a vertebrate cell) by using the above protein; a method of modifying the function of a cell by modifying the gene structure thereof; and a cell having been modified in function by these methods.
Also the structure of a cis element necessary in gene transfer is clarified and presented.
Scope of claims [claim1]
1. An isolated protein having an amino acid sequence as set forth in SEQ ID NO: 2.
[claim2]
2. The isolated protein of claim 1, wherein the protein catalyzes transposition of a Tol2 transposon.
[claim3]
3. The isolated protein of claim 1, which is encoded by a nucleotide sequence as set forth in SEQ ID NO: 1.
  • Inventor, and Inventor/Applicant
  • KAWAKAMI KOICHI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
U.S. Cl./(Sub)
  • 530/350
  • 536/23.1
  • 536/23.5
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