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Transposase and method of gene modification achieved

Foreign code F110005676
File No. Y0013US2
Posted date Sep 9, 2011
Country United States of America
Application number 37580406
Gazette No. 20060211116
Gazette No. 7468430
Date of filing Mar 14, 2006
Gazette Date Sep 21, 2006
Gazette Date Dec 23, 2008
International application number JP2000008014
International publication number WO2001040477
Date of international filing Nov 14, 2000
Date of international publication Jun 7, 2001
Priority data
  • P1999-345508 (Dec 3, 1999) JP
  • P2000-109033 (Apr 11, 2000) JP
  • 2000WO-JP08014 (Nov 14, 2000) WO
  • 2002US-10148639 (Jun 3, 2002) US
Title Transposase and method of gene modification achieved
Abstract (US7468430)
A transposase encoded by the Tol2 element; a polynucleotide encoding the same; a method of modifying the gene structure of a cell (preferably a vertebrate cell) by using the above protein; a method of modifying the function of a cell by modifying the gene structure thereof; and a cell having been modified in function by these methods.
Also the structure of a cis element necessary in gene transfer is clarified and presented.
Scope of claims [claim1]
1. An isolated nucleic acid encoding a protein having a transposase-like activity which can insert a fragment of a gene into the genome, wherein the protein consists of the amino acid sequence of SEQ ID NO: 2.
[claim2]
2. The nucleic acid according to claim 1, wherein the nucleic acid is DNA consisting of the nucleotide sequence of SEQ ID NO: 1.
[claim3]
3. The nucleic acid according to claim 1, wherein the nucleic acid is RNA.
  • Inventor, and Inventor/Applicant
  • KAWAKAMI KOICHI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
U.S. Cl./(Sub)
  • A01K067/027M
  • C12N009/22
  • C12N015/85A
  • C12N015/90
  • K01K217/05
  • K01K217/07B
  • K01K227/40
  • K61K038/00
  • K61K048/00
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