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Transposase and method of gene modification achieved

Foreign code F110005677
File No. Y0013US3
Posted date Sep 9, 2011
Country United States of America
Application number 37626406
Gazette No. 20060212959
Gazette No. 7741302
Date of filing Mar 14, 2006
Gazette Date Sep 21, 2006
Gazette Date Jun 22, 2010
International application number JP2000008014
International publication number WO2001040477
Date of international filing Nov 14, 2000
Date of international publication Jun 7, 2001
Priority data
  • P1999-345508 (Dec 3, 1999) JP
  • P2000-109033 (Apr 11, 2000) JP
  • 2000WO-JP08014 (Nov 14, 2000) WO
  • 2002US-10148639 (Jun 3, 2002) US
Title Transposase and method of gene modification achieved
Abstract (US7741302)
A transposase encoded by the Tol2 element; a polynucleotide encoding the same; a method of modifying the gene structure of a cell (preferably a vertebrate cell) by using the above protein; a method of modifying the function of a cell by modifying the gene structure thereof; and a cell having been modified in function by these methods.
Also the structure of a cis element necessary in gene transfer is clarified and presented.
Scope of claims [claim1]
1. A method of modifying a genome structure of a cell, the method comprising a) introducing a protein having the amino acid sequence of SEQ ID NO: 2, and a polynucleotide sequence comprising an exogenous nucleic acid and a cis-element of Oryzias Latipes Tol2 element into the cell, and b) modifying the genome structure by the insertion of the exogenous nucleic acid into the genome.
[claim2]
2. The method according to claim 1, wherein the cell is a vertebrate cell.
[claim3]
3. The method according to claim 1, wherein the exogenous nucleic acid and the cis-element of Oryzias Latipes Tol2 element are contained in a plasmid.
[claim4]
4. The method of claim 1, wherein the protein having the amino acid sequence of SEQ ID NO: 2 and the polynucleotide sequence of step a) are separately introduced into the cell.
[claim5]
5. The method of claim 1, wherein the protein having the amino acid sequence of SEQ ID NO: 2 and the polynucleotide sequence of step a) are simultaneously introduced into the cell.
  • Inventor, and Inventor/Applicant
  • KAWAKAMI KOICHI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
U.S. Cl./(Sub)
  • A01K067/027M
  • C12N009/22
  • C12N015/85A
  • C12N015/90
  • K01K217/05
  • K01K217/07B
  • K01K227/40
  • K61K038/00
  • K61K048/00
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