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Method and kit for detecting biological signal of three-dimensional cell culture material

Foreign code F110005841
File No. K02807WO
Posted date Oct 27, 2011
Country China
Application number 200980144966
Gazette No. 102209788
Gazette No. 102209788
Date of filing Nov 10, 2009
Gazette Date Oct 5, 2011
Gazette Date Jan 20, 2016
International application number JP2009069124
International publication number WO2010055829
Date of international filing Nov 10, 2009
Date of international publication May 20, 2010
Priority data
  • 2009JP069124 (Nov 10, 2009) WO
  • P2008-289011 (Nov 11, 2008) JP
Title Method and kit for detecting biological signal of three-dimensional cell culture material
Abstract Disclosed is a method for detecting a biological signal of a three-dimensional cell culture material.
The method for detecting a biological signal of a three-dimensional cell culture material comprises the steps of: providing a three-dimensional cell culture material comprising a cell layer composed of at least two layers laminated on each other and sensor particles capable of detecting the biological signal; and observing the sensor particles optically.
Preferably, the three-dimensional cell culture material comprises an extracellular matrix comprising a combination of a protein or polymer having an RGD sequence and a protein or polymer capable of interacting with the protein or polymer having the RGD sequence or a combination of a positively-charged protein or polymer and a negatively-charged protein or polymer.
  • Applicant
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • MICHIYA MATSUSAKI
  • MITSURU AKASHI
  • KAZUNORI OKANO
IPC(International Patent Classification)
Reference ( R and D project ) PRESTO Structures and control of interfaces AREA
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