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USE OF GENE INVOLVED IN ACCUMULATION OF CADMIUM IN PLANTS

Foreign code F110005863
File No. S2011-0544-N0
Posted date Nov 4, 2011
Country WIPO
International application number 2010JP065173
International publication number WO 2011030724
Date of international filing Sep 3, 2010
Date of international publication Mar 17, 2011
Priority data
  • P2009-208541 (Sep 9, 2009) JP
Title USE OF GENE INVOLVED IN ACCUMULATION OF CADMIUM IN PLANTS
Abstract The purposes of the present invention are to identify a gene involved in accumulation of cadmium in plants and to provide a method for using the gene. Specifically, a polynucleotide encoding a polypeptide which comprises the amino acid sequence represented by SEQ ID NO: 1, a polynucleotide encoding a polypeptide which comprises an amino acid sequence produced by substituting, deleting and/or adding one or several amino acids in the amino acid sequence represented by SEQ ID NO: 1 and has an activity of changing the localization of the accumulation of cadmium, a polynucleotide encoding a polypeptide which comprises the amino acid sequence represented by SEQ ID NO: 2, or a polynucleotide encoding a polypeptide which comprises an amino acid sequence produced by substituting, deleting and/or adding one or several amino acids in the amino acid sequence represented by SEQ ID NO: 2 and has an activity of changing the localization of the accumulation of cadmium is introduced into a plant such that the polynucleotide is able to be expressed.
Outline of related art and contending technology BACKGROUND ART
The causative agent of cadmium is known as itai-itai disease, toxic heavy metals. Cadmium is, during the mineral naturally present in the soil or heavy metals, silver, copper, the presence of the same with metals such as zinc, mining of these metals, and refining the discharged into the environment as a byproduct when accumulated in the soil.
Cadmium contaminated soil cultivated vegetables, cereal crops via various cadmium is taken into the human body, our with damaging consequences to health. For this reason, in Japan is to limit the amount of intake of cadmium from agricultural crops, by with a method, the reference value of the crop is included in the cadmium concentration determined. For example, 0.4 ppm or more the concentration of cadmium in the brown rice of less than 1.0 ppm of an edible rice marketed inhibited, the concentration of 1 ppm or more of the rice is cadmium, or the like of a sales processing, incineration being disposed as an entity.
Included in the rice crop such as cadmium concentration equal to or less than the reference value, in order to ensure the safety of the diet, conventional, soil washing, landfill, soil dressing by a method such as cadmium contaminated soil purification attempt has been made (for example, Patent Document 1 and see non-patent document 1).
Scope of claims (In Japanese)請求の範囲 [請求項1]
 カドミウムの蓄積の局在性を変化させた形質転換植物の生産方法であって、
 下記の(a)~(d)のいずれかのポリヌクレオチドを、植物に発現可能に導入することを特徴とする、形質転換植物の生産方法:
 (a)配列番号1に示すアミノ酸配列からなるポリペプチドをコードするポリヌクレオチド;
 (b)配列番号1に示すアミノ酸配列において、1個または数個のアミノ酸が置換、欠失、および/または付加されたアミノ酸配列からなり、且つカドミウムの蓄積の局在性を変化させる活性を有するポリペプチドをコードするポリヌクレオチド;
 (c)配列番号2に示すアミノ酸配列からなるポリペプチドをコードするポリヌクレオチド;
 (d)配列番号2に示すアミノ酸配列において、1個または数個のアミノ酸が置換、欠失、および/または付加されたアミノ酸配列からなり、且つカドミウムの蓄積の局在性を変化させる活性を有するポリペプチドをコードするポリヌクレオチド。

[請求項2]
 上記植物はイネ科植物であることを特徴とする請求項1に記載の形質転換植物の生産方法。

[請求項3]
 請求項1または2に記載の形質転換植物の生産方法によって生産されたことを特徴とする形質転換植物。

[請求項4]
 カドミウムの蓄積の局在性を変化させた形質転換植物を作製するためのキットであって、
 下記の(a)~(d)のいずれかのポリヌクレオチドを備えていることを特徴とするキット:
 (a)配列番号1に示すアミノ酸配列からなるポリペプチドをコードするポリヌクレオチド;
 (b)配列番号1に示すアミノ酸配列において、1個または数個のアミノ酸が置換、欠失、および/または付加されたアミノ酸配列からなり、且つカドミウムの蓄積の局在性を変化させる活性を有するポリペプチドをコードするポリヌクレオチド;
 (c)配列番号2に示すアミノ酸配列からなるポリペプチドをコードするポリヌクレオチド;
 (d)配列番号2に示すアミノ酸配列において、1個または数個のアミノ酸が置換、欠失、および/または付加されたアミノ酸配列からなり、且つカドミウムの蓄積の局在性を変化させる活性を有するポリペプチドをコードするポリヌクレオチド。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NATIONAL UNIVERSITY CORPORATION OKAYAMA UNIVERSITY
  • Inventor
  • MA, Jian Feng
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT RO RS RU SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

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