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FLOWERING INDUCER

Foreign code F110006071
File No. S2010-0101
Posted date Dec 28, 2011
Country WIPO
International application number 2010JP070181
International publication number WO 2011059051
Date of international filing Nov 12, 2010
Date of international publication May 19, 2011
Priority data
  • P2009-260847 (Nov 16, 2009) JP
Title FLOWERING INDUCER
Abstract Disclosed is a flowering inducer comprising any one protein selected from the following proteins (A) to (C): (A) a protein that comprises the amino acid sequence represented by SEQ ID NO:2; (B) a protein that comprises an amino acid sequence produced by deleting, substituting and/or adding one or a few amino acid residues in the amino acid sequence represented by SEQ ID NO:2 and has a flowering-inducing activity; and (C) a protein that comprises an amino acid sequence having a 60% or more identity with the amino acid represented by SEQ ID NO:2 and has a flowering-inducing activity. The flowering inducer can be administered to a plant from the outside of the plant.
Outline of related art and contending technology BACKGROUND ART
Such as a fruit tree crizzles , the number of years has not yet elapsed after germination floral constant not wearing. With respect to the reproductive organs such as flowers, leaves and stems can be referred to as organ of nutrient, in a period of no wearing a flower, a fruit tree, the vegetative growth to continue only vegetative organs. This vegetative growth is referred to as juvenile phase continues to. Characterized in that the seedling is crossed crizzles, juvenile phase is up to 8-12 years. After the juvenile stage, mature period begins. This maturation period, a fruit tree, reproductive growth (words for vegetative growth, flowering, such as reproductive related crop is referred to as the germ growth growth processes.) And, flowering occurs.
In cultivated plants, often the grafting is performed. One of the reasons is performed , a fruit tree has a long juvenile period in order to overcome (non-patent document 1: Nobuhito Mitani, Ryoji Matsumoto, Terutaka Yoshioka, and Takeshi Kuniga (2008) "Citrus hybrid seedlings reduce initial time to flower when grafted onto shiikuwasha rootstock"Scientia Horticulturae 116, 452-455.). In the case of one of the 1 , of to, the ears of the tree of maturity, the grafting is often performed. In this case, the ears of the tree is, in the stage of ripening. Therefore, the ears of the tree is, the original, with the formation of flower buds ability. However, the ears of the tree is, only the vegetative growth for a while after the grafting, there is no formation of flower buds. The chain portion (portion of the seedling ) tree of the vegetative growth period, referred to as nutritional maturity. 4 Followed by a stage of ripening is nutritionally generally about ten years. However, the cost reduction of the cultivation, the costs of recovery and early charged from the viewpoint of the efficiency of the breeding, nutritionally the development of a method of reducing the maturity has been demanded.
Here, as to a gene involved in flowering, 'T(Flowering Locus T) 'flowering locus of the gene (hereinafter, ' ft gene ' that) has been known. This gene encodes an ft proteins, referred to as ft protein. As shown in Fig. 1, ft protein is, according to the change in the environment such as leaves after the synthesis, is shifted to the fibroblast growth factor, to induce the formation of flower buds is already determined (non-patent document 2: Laurent Corbesier, Coral Vincent, Seonghoe Jang, Fabio Fornara, Qingzhi Fan, Iain Searle, Antonis Giakountis, Sara Farrona, Lionel Gissot, Colin Turnbull, and George Coupland (2007) "FT Protein Movement Contributes to Long-Distance Signaling in Floral Induction of Arabidopsis"Science 316, 1030-1033.). In this way, are combined and leaves, is shifted to the fibroblast growth factor, a substance which induces the formation of flower buds are referred to as hormone formation of flowers, flower formation ft protein is thought to be the main body of the hormone.
A gene from Arabidopsis thaliana ft, at an early flowering Arabidopsis are studied. For example, Patent Document 1 (Japanese Patent Application JP-2002-511270) of the method, the plant body of a ft protein is highly expressed in order to, the cauliflower mosaic virus gene behind the 35S promoter including connecting ft DNA fragment was introduced into Arabidopsis thaliana, is generated using the transgenic Arabidopsis thaliana. As a result, premature flowering time of the obtained transgenic Arabidopsis thaliana.
Is of a similar study, have also been carried out in the can (non-patent document 3: Tomoko Endo, Takehiko Shimada, Hiroshi Fujii, Yasushi Kobayashi, Takashi Araki, and Mitsuo Omura (2005) "Ectopic expression of an FThomolog from citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.) "Transgenic Research 14, 703-712.). In this study Arabidopsis (Citrus unshiu Marc.) ft gene derived from a high homology with the gene CiFT is used. In this method, the cauliflower mosaic virus gene behind the 35S promoter including CiFT connecting (Poncirus trifoliata) DNA is introduced into the fragment, is generated using the gene recombinant . As a result, the timing of flowering of premature are. More specifically, on the appearance of the hypocotyl, flowering in minimum half year is being viewed.
These prior art is, using both the transgenic plant, ft protein (such as the above CiFT protein, protein Arabidopsis ft comprising high homology with the protein, as used herein, simply 'ft protein' may be referred to.) Are over-expressed in the plant body. However, is a method of using the transgenic plant, it should be noted that some of the matter.
One, the early flowering transgenic plant sometimes. Transgenic plant since there are many restrictions is legal, the plant or the transgenic plant in the field to be ready for the, time is required. In addition, the transgenic plant of the consumer to be understood that a current state does not proceed.
The other is, very young plant, flower buds in some cases (non-patent document 3: Tomoko Endo, Takehiko Shimada, Hiroshi Fujii, Yasushi Kobayashi, Takashi Araki, and Mitsuo Omura (2005) "Ectopic expression of an FT homolog from citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.) "Transgenic Research 14, 703-712.). Can be the fruit, leaf growth, and thus inhibit the growth of the plant body of a subject. Therefore, the formation of flower buds is too fast, so that a large burden for young plants, fruit and for the growth of the plants for the production of which is not.
Scope of claims (In Japanese)請求の範囲 [請求項1]
 以下の(A)~(C)のいずれかのタンパク質を含む、開花誘導剤。
(A)配列番号2記載のアミノ酸配列を含むタンパク質
(B)配列番号2記載のアミノ酸配列において、1もしくは数個のアミノ酸が欠失、置換および/または付加されたアミノ酸配列を含み、かつ開花誘導活性を有するタンパク質
(C)配列番号2記載のアミノ酸配列と60%以上の同一性を有するアミノ酸配列を含み、かつ開花誘導活性を有するタンパク質

[請求項2]
 前記(C)のタンパク質が、配列番号2記載のアミノ酸配列と95%以上の同一性を有するアミノ酸配列を含み、かつ開花誘導活性を有するタンパク質である、請求項1記載の開花誘導剤。

[請求項3]
 以下の(a)~(c)のいずれかのDNAを含む遺伝子によりコードされるタンパク質を含む、開花誘導剤。
(a)配列番号1記載の塩基配列からなるDNA
(b)配列番号1記載の塩基配列に相補的な塩基配列からなるDNAとストリンジェントな条件下でハイブリダイズし、かつ開花誘導活性を有するタンパク質をコードするDNA
(c)配列番号1記載の塩基配列と60%以上の同一性を有する塩基配列を含み、かつ開花誘導活性を有するタンパク質をコードするDNA

[請求項4]
 前記(c)のDNAが、配列番号1記載の塩基配列と95%以上の同一性を有する塩基配列を含み、かつ開花誘導活性を有するタンパク質をコードするDNAである、請求項3記載の開花誘導剤。

[請求項5]
 配列番号4記載のアミノ酸配列からなるタンパク質を含む、請求項1記載の開花誘導剤。

[請求項6]
 配列番号3記載の塩基配列からなるDNAを含む遺伝子によりコードされるタンパク質を含む、請求項3記載の開花誘導剤。

[請求項7]
 果樹の開花誘導に用いられる、請求項1~6のいずれか1項に記載の開花誘導剤。

[請求項8]
 果樹がカンキツである、請求項7記載の開花誘導剤。

[請求項9]
 カンキツがウンシュウミカンである、請求項8記載の開花誘導剤。

[請求項10]
 以下の(A)~(C)のいずれかのタンパク質を用いて植物を処理することを特徴とする開花誘導方法。
(A)配列番号2記載のアミノ酸配列を含むタンパク質
(B)配列番号2記載のアミノ酸配列において、1もしくは数個のアミノ酸が欠失、置換および/または付加されたアミノ酸配列を含み、かつ開花誘導活性を有するタンパク質
(C)配列番号2記載のアミノ酸配列と60%以上の同一性を有するアミノ酸配列を含み、かつ開花誘導活性を有するタンパク質

[請求項11]
 前記(C)のタンパク質が、配列番号2記載のアミノ酸配列と95%以上の同一性を有するアミノ酸配列を含み、かつ開花誘導活性を有するタンパク質である、請求項10記載の方法。

[請求項12]
 以下の(a)~(c)のいずれかのDNAを含む遺伝子によりコードされるタンパク質を用いて植物を処理することを特徴とする開花誘導方法。
(a)配列番号1記載の塩基配列からなるDNA
(b)配列番号1記載の塩基配列に相補的な塩基配列からなるDNAとストリンジェントな条件下でハイブリダイズし、かつ開花誘導活性を有するタンパク質をコードするDNA
(c)配列番号1記載の塩基配列と60%以上の同一性を有する塩基配列を含み、かつ開花誘導活性を有するタンパク質をコードするDNA

[請求項13]
 前記(c)のDNAが、配列番号1記載の塩基配列と95%以上の同一性を有する塩基配列を含み、かつ開花誘導活性を有するタンパク質をコードするDNAである、請求項12記載の方法。

[請求項14]
 配列番号4記載のアミノ酸配列からなるタンパク質を用いて植物を処理する、請求項10記載の方法。

[請求項15]
 配列番号3記載の塩基配列からなるDNAを含む遺伝子によりコードされるタンパク質を用いて植物を処理する、請求項12記載の方法。

[請求項16]
 前記タンパク質が、果樹の開花誘導に用いられる、請求項10~15のいずれか1項に記載の方法。

[請求項17]
 果樹がカンキツである、請求項16記載の方法。

[請求項18]
 カンキツがウンシュウミカンである、請求項17記載の方法。

[請求項19]
 植物が、成熟相にある穂木を台木に接ぎ木した植物である、請求項10~18のいずれか1項に記載の方法。

[請求項20]
 前記タンパク質を投与した植物を、開花誘導の目的の植物の隣に配置することを含む、請求項10~19のいずれか1項に記載の方法。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • SAGA UNIVERSITY
  • Inventor
  • MATSUMOTO Ryoji
  • NAGANO Yukio
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT RO RS RU SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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