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Light-responsive artificial nucleotide having photo-crosslinking ability

Foreign code F120006086
File No. S2008-0144
Posted date Jan 6, 2012
Country United States of America
Application number 74332408
Gazette No. 20100274000
Gazette No. 8481714
Date of filing Nov 19, 2008
Gazette Date Oct 28, 2010
Gazette Date Jul 9, 2013
International application number JP2008003376
International publication number WO2009066447
Date of international filing Nov 19, 2008
Date of international publication May 28, 2009
Priority data
  • P2007-299914 (Nov 19, 2007) JP
  • 2008WO-JP03376 (Nov 19, 2008) WO
Title Light-responsive artificial nucleotide having photo-crosslinking ability
Abstract (US8481714)
The present invention provides a photoreactive crosslinking agent that is capable of crosslinking a sequence which cannot be photo-crosslinked by psoralen, and is capable of photo-crosslinking using a light having a longer wavelength, as compared with psoralen.
The present invention also provides a compound having a group represented by formula (I) coupled with a group represented by formula (II).
Scope of claims [claim1]
1. An agent comprising: a compound represented by the following formula (V)
wherein, in the formula (V), Ra represents a cyano group, an amide group, a carboxyl group, a C2-C7 alkoxycarbonyl group, or hydrogen,
wherein, in the formula (V), R1 and R2 each independently represent a cyano group, an amide group, a carboxyl group, a C2-C7 alkoxycarbonyl group, or hydrogen, and
wherein, in the formula (V), Rb represents a sugar, a polysaccharide, a polyether, a polyol, a polypeptide chain, or a water-soluble synthetic polymer.
[claim2]
2. The agent according to claim 1, wherein Rb is represented by the following formula (III) or formula (IV):
[claim3]
3. The agent according to claim 1, wherein the compound is a nucleoside.
[claim4]
4. The agent according to claim 1, wherein the compound is a nucleotide.
[claim5]
5. The agent according to claim 1, wherein the compound is a nucleic acid.
[claim6]
6. The agent according to claim 1, wherein Rb is represented by the following formula (IX) or formula (X):
[claim7]
7. The agent according to claim 6, wherein the compound is represented by one of the following formulae:
[claim8]
8. A method of forming a crosslink, comprising: providing a compound and a nucleobase having a pyrimidine ring, and
irradiating the compound and the nucleobase with light,
wherein the compound is represented by the following formula (V):
wherein, in the formula (V), Ra represents a cyano group, an amide group, a carboxyl group, a C2-C7 alkoxycarbonyl group, or hydrogen,
wherein, in the formula (V), R1 and R2 each independently represent a cyano group, an amide group, a carboxyl group, a C2-C7 alkoxycarbonyl group, or hydrogen, and
wherein, in the formula (V), Rb represents a sugar, a polysaccharide, a polyether, a polyol, a polypeptide chain, or a water-soluble synthetic polymer.
[claim9]
9. The method of claim 8, wherein Rb is represented by the following formula (III) or formula (IV):
[claim10]
10. A method for producing a nucleic acid, comprising: synthesizing a nucleic acid using the compound of claim 6 as a synthesizing reagent.
[claim11]
11. A method for producing a nucleic acid, comprising: synthesizing a nucleic acid using the compound of claim 7 as a synthesizing reagent.
  • Inventor, and Inventor/Applicant
  • FUJIMOTO KENZO
  • YOSHIMURA YOSHINAGA
  • TOBA SHINYA
  • NITTA YUKARI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
IPC(International Patent Classification)
U.S. Cl./(Sub)
  • C07D209/86
  • C07D405/04
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