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L-cysteine-producing bacterium and a method for producing L-cysteine

外国特許コード F120006097
整理番号 S2008-0346
掲載日 2012年1月6日
出願国 アメリカ合衆国
出願番号 85859010
公報番号 20110033902
公報番号 8278075
出願日 平成22年8月18日(2010.8.18)
公報発行日 平成23年2月10日(2011.2.10)
公報発行日 平成24年10月2日(2012.10.2)
優先権データ
  • 特願2008-040167 (2008.2.21) JP
  • 2009WO-JP53021 (2009.2.20) WO
発明の名称 (英語) L-cysteine-producing bacterium and a method for producing L-cysteine
発明の概要(英語) (US8278075)
L-Cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof is produced by culturing a bacterium belonging to the family Enterobacteriaceae, which has L-cysteine-producing ability and has been modified so that the activity of a protein encoded by a tolC gene, for example, a protein defined in the following (a) or (b), is increased in a medium, and by collecting L-cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof from the medium: (a) a protein comprising the amino acid sequence of SEQ ID NO: 2, (b) a protein comprising the amino acid sequence of SEQ ID NO: 2, but wherein one or several amino acid residues are substituted, deleted, inserted or added, increase of which activity in the bacterium improves the ability of the bacterium to produce L-cysteine.
特許請求の範囲(英語) [claim1]
1. A method for producing L-cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof, which comprises culturing a bacterium belonging to the family Enterobacteriaceae in a medium and collecting L-cysteine, L-cystine, a derivative or precursor thereof, or a mixture thereof from the medium, wherein the bacterium has the ability to produce L-cysteine and has been modified so that an activity of a protein encoded by a tolC gene is increased as compared to a non-modified bacterium, wherein the activity of the protein is increased by increasing expression amount of the tolC gene, increasing translation amount of the tolC gene, or combinations thereof.
[claim2]
2. The method according to claim 1, wherein the derivative of L-cysteine is a thiazolidine derivative.
[claim3]
3. The method according to claim 1, wherein the precursor of L-cysteine is O-acetylserine or N-acetylserine.
[claim4]
4. The method according to claim 1, wherein expression amount of the tolC gene is increased by increasing a copy number of the tolC gene, or by modifying an expression control sequence of the gene.
[claim5]
5. The method according to claim 1, wherein the protein is selected from the group consisting of: (a) a protein comprising the amino acid sequence of SEQ ID NO: 2, and
(b) a protein comprising the amino acid sequence of SEQ ID NO: 2, but wherein one to five amino acid residues are substituted, deleted, inserted or added, wherein the increase of the activity in the bacterium improves the ability of the bacterium to produce L-cysteine.
[claim6]
6. The method according to claim 1, wherein the tolC gene is selected from a group consisting of: (a) a DNA comprising the nucleotide sequence of SEQ ID NO: 1,
(b) a DNA which hybridizes with the nucleotide sequence of SEQ ID NO: 1, or a probe prepared from the nucleotide sequence, under stringent conditions comprising washing at 60 deg. C. at a salt concentration of 0.1 * SSC and 0.1% SDS, and codes for a protein, wherein the increase of the activity in the bacterium improves the ability of the bacterium to produce L-cysteine.
[claim7]
7. The method according to claim 1, wherein the bacterium contains a mutant serine acetyltransferase in which feedback inhibition by L-cysteine has been attenuated.
[claim8]
8. The method according to claim 1, wherein the bacterium has increased activity of the protein encoded by a ydeD gene.
[claim9]
9. The method according to claim 1, wherein the bacterium has decreased activity of a protein having cysteine desulfhydrase activity.
[claim10]
10. The method according to claim 7, wherein the bacterium has increased activity of the protein encoded by a ydeD gene.
[claim11]
11. The method according to claim 7, wherein the bacterium has decreased activity of a protein having cysteine desulfhydrase activity.
[claim12]
12. The method according to claim 8, wherein the bacterium has decreased activity of a protein having cysteine desulfhydrase activity.
[claim13]
13. The method according to claim 10, wherein the bacterium has decreased activity of a protein having cysteine desulfhydrase activity.
[claim14]
14. The method according to claim 9, wherein the protein having the cysteine desulfhydrase activity is tryptophanase.
[claim15]
15. The method according to claim 1, wherein the bacterium is an Escherichia bacterium.
[claim16]
16. The method according to claim 15, wherein the bacterium is Escherichia coli.
  • 発明者/出願人(英語)
  • NONAKA GEN
  • TAKAGI HIROSHI
  • OHTSU IWAO
  • AJINOMOTO
  • NARA INSTITUTE OF SCIENCE AND TECHNOLOGY
国際特許分類(IPC)
米国特許分類/主・副
  • C07K014/245
  • C12N001/20
  • C12P013/12
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