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METHOD FOR DECOMPOSING ALGINIC ACID meetings

Foreign code F120006262
File No. SHINGI20120228
Posted date Feb 24, 2012
Country WIPO
International application number 2011JP056835
International publication number WO 2011118582
Date of international filing Mar 22, 2011
Date of international publication Sep 29, 2011
Priority data
  • P2010-065945 (Mar 23, 2010) JP
Title METHOD FOR DECOMPOSING ALGINIC ACID meetings
Abstract Disclosed are a polypeptide having alginate lyase activity, and a method that is for decomposing alginic acid and that uses said enzyme.
Outline of related art and contending technology BACKGROUND ART
Brown algae such as alginic acid-producing bacteria and a portion of algae polysaccharides, in particular Laminaria such as alcohol or a contained many . Alginic acid is directly extracted from brown algae as well as a food, food additive, cosmetic, pharmaceutical, industrial are utilized in various other applications. On the other hand, alginate is soluble in water for very high viscosity, food, food additive, cosmetic, pharmaceutical, industrial in order to be applied to various purposes such as, by the decomposition of the alginate lyase by alginic acid, alginic acid to reduce the viscosity of the aqueous solution is effective. In addition, alginic acid oligosaccharide is created by the decomposition of the roots of the plant growth-promoting effect, such as anti-tumor effect in a variety of physiologic activating action have been reported, has attracted attention in recent years. In addition, food processing plant using brown algae is the large amount of the wastewater in the alginic acid exhibits a high viscosity, the construction of the alginic acid decomposition by a viscous size reduction techniques are also demanded. Further, such as the genus Pseudomonus of alginate produced as the removal of the biofilm bacteria, as well as surfactant and heat treatment is difficult, the decomposition of the alginate are considered essential.
Alginic acid and decomposition of the large amount of energy consumption or a waste liquid processing is complicated due to heat method than the method according to the acid, using enzymes have been considered valid. The ocean to the land-based or live bacteria mainly brown algae or marine mollusks to the feed intake (oysters, , snail and the like) to the subject a degradative enzyme alginate alginate lyase and a search is performed, the substrate specificity of the enzyme, a truncated version of the characteristics such as discussed in detail in, for some enzymes are the three-dimensional structure is revealed. In addition, those enzymes from brown algae or the production of oligosaccharides is protoplast cells has been attempted to apply the polymer to the production of the practical example there is little. In this way decomposition and alginate oligosaccharide is a degradation product useful, mainly degradative enzyme alginate lyase is derived from a land-based soil bacteria are commercially available and has only a small.
Alginic acid degrading enzyme is not limited to, industrial and industrial enzyme generally is used, it is important to the stability of the enzyme. Temperature, pH, to various factors such as ionic strength, and to keep the enzymatic activity, does not easily inactivated or degeneration is required. For example, sugars such as cellulase and a protease enzyme hydrolysis habitat of various fungi that produce them from isolated, useful enzymes are found in practical use. However, the hitherto known alginate lyase to produce the biological species may be small compared to the above-mentioned enzymes for, order for practical use of alginic acid which overcomes the various factors for degrading enzyme has scarcely been reported.
Alginic acid lyase producing organism up to now is mainly used as soil bacteria, seaweed, seaweed attached bacteria, algae and the like are covered mollusk meat has been (non-patent document 1-6).
Scope of claims (In Japanese)請求の範囲 [請求項1]
 配列番号1のアミノ酸配列からなるか、または配列番号1のアミノ酸配列において1もしくは数個のアミノ酸が欠失、置換もしくは付加されたアミノ酸配列からなり、かつアルギン酸リアーゼ活性を有するポリペプチド。

[請求項2]
 配列番号1のアミノ酸配列と20%以上の同一性を有するアミノ酸配列において、配列番号1のアミノ酸配列の80位および232位に相当する位置にシステイン残基を有するアミノ酸配列からなり、かつアルギン酸リアーゼ活性を有するポリペプチド。

[請求項3]
 配列番号1のアミノ酸配列と20%以上の同一性を有するアミノ酸配列において、配列番号1のアミノ酸配列の80位および/または232位に相当する位置におけるシステイン残基以外のアミノ酸残基をシステイン残基に置換して、これらの位置の両方にシステイン残基が存在するようにすることを含む、温度特性が向上したアルギン酸リアーゼ活性を有するポリペプチドの製造方法。

[請求項4]
 請求項1または2記載のポリペプチドをアルギン酸に作用させることを含む、アルギン酸の分解方法。

[請求項5]
 請求項1または2記載のポリペプチドを含む、アルギン酸分解用組成物。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
  • Inventor
  • INOUE, Akira
  • NAKAGAWA, Satoshi
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT RO RS RU SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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