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METHOD FOR PREPARING POLYPEPTIDE LIBRARY meetings

Foreign code F120006264
File No. SHINGI20120224
Posted date Feb 24, 2012
Country WIPO
International application number 2010JP054009
International publication number WO 2010/104114
Date of international filing Mar 10, 2010
Date of international publication Sep 16, 2010
Priority data
  • P2009-057114 (Mar 10, 2009) JP
Title METHOD FOR PREPARING POLYPEPTIDE LIBRARY meetings
Abstract Provided is a polypeptide library for identifying a polypeptide that has a high affinity for a desired target. The polypeptide library has a scaffold, which has a relatively low molecular weight and carries a small number of cysteine residues, is applicable to a high throughput screening system targeting a membrane protein, and is characterized by consisting of a plurality of polypeptides having an ICK scaffold, and the amino acid sequence of each of the aforesaid polypeptides comprising an amino acid sequence having been simultaneously randomized in the following regions (i) to (iv): (i) the amino acid sequence from the fourth position from the C-terminus of Cys-1 to the first position from the N-terminus of Cys-2 in the loop I region between Cys-1 and Cys-2; (ii) the amino acid sequence from the third position from the C-terminus of Cys-2 to the first position from the N-terminus of Cys-3 in the loop II region between Cys-2 and Cys-3; (iii) the amino acid sequence from the second position from the C-terminus of Cys-5 to the third position from the N-terminus of Cys-6 in the loop III region between Cys-5 and Cys-6; and (iv) the amino acid sequence from the third position from the C-terminus of Cys-6 to the C-terminus in the tail region from Cys-6 to the C-terminus.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
  • Inventor
  • KUBO Tai
  • KIMURA Tadashi
  • ONO Seigo
IPC(International Patent Classification)

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