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VACCINE FOR CRUSTACEAN ACUTE VIRAEMIA

Foreign code F120006285
File No. A-021
Posted date Mar 6, 2012
Country WIPO
International application number 2010JP060503
International publication number WO 2011004697
Date of international filing Jun 22, 2010
Date of international publication Jan 13, 2011
Priority data
  • P2009-162802 (Jul 9, 2009) JP
Title VACCINE FOR CRUSTACEAN ACUTE VIRAEMIA
Abstract Disclosed is a vaccine effective for the prevention of crustacean acute viraemia. Specifically disclosed is a vaccine for preventing crustacean acute viraemia, which utilizes VP28 protein encoded by VP28 gene of a white-spot syndrome virus (WSSV) as an antigen.
Outline of related art and contending technology BACKGROUND ART
Acute viremia of crustaceans (white spot syndrome, also referred to as diseases such as vitiligo) is, the most severe damage in farming of shellfish is viral disease have been reported. This disease is shown in a high lethality for several days from infection, resulting in effective prevention, treatment method has not been established. Therefore, the pathogenic virus is white spot syndrome virus (White Spot Syndrome Virus; WSSV) not brought into the farm, only this disease with respect to the effectiveness of the current being a measure. However, once the pathogenic virus and from entering in the farm, in order to clear the virus which are raised on only discards all crustaceans, very large economic losses. In this pathogenic virus WSSV is, has a wide host range crustaceans (non-patent document 1), genetic variation between isolates also are hardly observed (non-patent document 2) from, one 1 is at a close WSSV infection in crustaceans cultured expanded at once to a different type of crustaceans is a possibility that the high. Therefore, this disease convenience for the prevention of the development of more effectively been desired earnestly.
In the crustacean viral disease as a prophylactic, Patent Document 1 and 2 is, the structural proteins of WSSV as a vaccine, or encoding a structural protein such as a live attenuated bacterial or viral gene has been introduced as a vaccine, is administered a crustacean disclosed.
For aquatic animals as other antiviral agents, virus vector that encodes a protein as an active ingredient for promoting the development of vaccines has also been carried DNA (patent document 3). Does not have the infectious DNA vaccine, held for a long time in vivo humoral immune response and cell-mediated immune response that is capable of both, the manufacturing and storage is also easy. However DNA vaccine will be, by the method of administration to elicit an immune response cannot be effectively problems often arise.
In recent years, in order to enhance the immunostimulatory DNA in a vaccine, non-methylated CpG motif characteristic of bacteria (methylated cytosine and guanine-rich DNA sequence) is incorporated into a vector DNA as adjuvants known method (for example, the non-patent document 3 and patent document 3). In this method, non-methylated CpG motif is recognized by the TLR9 receptor, are drawn into a cell by the immune system, such as various inflammatory cytokine production of immune activation response is elicited, the immune response is believed to be enhanced. However non-methylated CpG motifs of immunostimulatory with an immunostimulatory sequence including for the function, many points are still unclear.
In order to solve the above-described problems, the inventors of the present invention, the gene encoding the viral structural proteins WSSV for mammalian expression vector is incorporated into the vector DNA made pTargeTTM DNA vaccine was administered to prawn, acute viremia WSSV very effectively prevent have found (patent document 4).
Scope of claims (In Japanese)請求の範囲 [請求項1]
 ホワイトスポットシンドロームウイルス(WSSV)のVP28遺伝子によりコードされるVP28タンパク質を抗原とした、甲殻類急性ウイルス血症予防用のワクチン。

[請求項2]
 上記VP28タンパク質は、無細胞タンパク質合成系で作製されたタンパク質であることを特徴とする請求項1記載のワクチン。

[請求項3]
 甲殻類がクルマエビ科である、請求項1又は2に記載のワクチン。

[請求項4]
 請求項1乃至3いずれか一項記載のワクチンを有効成分とする甲殻類急性ウイルス血症予防剤。

[請求項5]
 注射投与製剤である、請求項4記載の甲殻類急性ウイルス血症予防剤。

[請求項6]
 経口投与製剤である、請求項4記載の甲殻類急性ウイルス血症予防剤。

[請求項7]
 展着剤を更に含む、請求項6記載の甲殻類急性ウイルス血症予防剤。

[請求項8]
 請求項1~3のいずれか1項に記載のワクチンを甲殻類に投与することを特徴とする、甲殻類急性ウイルス血症を予防する方法。

[請求項9]
 甲殻類がクルマエビ科である、請求項8に記載の方法。

[請求項10]
 上記ワクチンを経口投与する、請求項8に記載の方法。

[請求項11]
 上記ワクチン及び展着剤を含む飼料を投与する、請求項8に記載の方法。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • UNIVERSITY OF MIYAZAKI
  • Inventor
  • SAKAI Masahiro
  • ITAMI Toshiaki
  • KONO Tomoya
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT RO RS RU SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

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