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MATERIAL FOR SCREENING FOR COMPOUND CAPABLE OF ACTING ON ION CHANNEL, AND USE THEREOF 新技術説明会

外国特許コード F120006593
整理番号 S2010-0739
掲載日 2012年5月15日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2011JP064967
国際公開番号 WO 2012002460
国際出願日 平成23年6月29日(2011.6.29)
国際公開日 平成24年1月5日(2012.1.5)
優先権データ
  • 特願2010-147255 (2010.6.29) JP
発明の名称 (英語) MATERIAL FOR SCREENING FOR COMPOUND CAPABLE OF ACTING ON ION CHANNEL, AND USE THEREOF 新技術説明会
発明の概要(英語) Disclosed is a screening system which targets an ion channel and has excellent efficiency. Specifically disclosed is a material for use in the screening of a compound capable of acting on a target ion channel, which comprises a cell that carries at least one first DNA encoding a voltage-dependent Na ion channel that cannot be inactivated, wherein a K ion channel in the cell is so activated that the depth of the resting membrane potential is increased gradually in the negative direction.
従来技術、競合技術の概要(英語) BACKGROUND ART
Ion channels are physiologically important has a function. As a target ion channel, exerting on the ion channel to search for an agent or inhibitor, useful agents are expected to provide. Such an ion channel, for example, drugs that target voltage dependent ion channel as the evaluation method of a screening system, a change in cell membrane potential in a voltage dependent fluorescent dye to detect fluorescent membrane potential measurement method is known (patent document 1). In addition, the glass with respect to electrode of a cell membrane and membrane potential (seal) is electrically detected also known patch-clamp method. Further, in recent years, corresponds to the tip end of the glass electrode in each well having an opening using , patch electrodes with the cell membrane in the wells of the seal film automatically performed by detecting the potential method has been developed (non-patent document 1) have been.
Of a conventional fluorescent membrane potential measuring method includes, but is amenable to evaluation of multiple specimens, the ion channel measurement accuracy or applicable range is limited in some cases. In addition, the patch-clamp method, high measurement accuracy, it is often obtained by measurement of the amount of information, number which can be measured at one time is small and the efficiency was low. Further, the method , multiple specimens simultaneously assess structure However, the success rate of the low patch, is not suitable for the high throughput screening. In addition, method, apparatus and high running cost was also a problem.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • Public University Corporation Nagoya City University
  • Channelo Search Technology
  • 発明者(英語)
  • IMAIZUMI Yuji
  • FUJII Masato
  • OHYA Susumu
  • YAMAMURA Hisao
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT RO RS RU SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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