TOP > 外国特許検索 > Oligonucleotide derivative, labeling agent and use for labeling agent

Oligonucleotide derivative, labeling agent and use for labeling agent

外国特許コード F120006738
整理番号 K02704WO
掲載日 2012年5月30日
出願国 アメリカ合衆国
出願番号 200913126320
公報番号 20120126175
公報番号 8354515
出願日 平成21年11月2日(2009.11.2)
公報発行日 平成24年5月24日(2012.5.24)
公報発行日 平成25年1月15日(2013.1.15)
国際出願番号 JP2009068785
国際公開番号 WO2010055789
国際出願日 平成21年11月2日(2009.11.2)
国際公開日 平成22年5月20日(2010.5.20)
優先権データ
  • 特願2008-292815 (2008.11.14) JP
  • 2009WO-JP68785 (2009.11.2) WO
発明の名称 (英語) Oligonucleotide derivative, labeling agent and use for labeling agent
発明の概要(英語) (US8354515)
A labeling agent comprising a plurality of nucleotide derivative units represented by Formula (1) below: (where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group, X1 represents O, S or Se, and X2 represents SH (or S-), Se-, a C1-4 alkyl or a morpholino group).
特許請求の範囲(英語) [claim1]
1. A labeling agent comprising a plurality of nucleotide derivative units represented by Formula (1) below:

(where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X1 represents O, S or Se, and X2 represents SH (or S-), Se-, or a C1-4 alkyl group or morpholino group).
[claim2]
2. The labeling agent according to claim 1, wherein said Y is a condensed polycyclic hydrocarbon group.
[claim3]
3. The labeling agent according to claim 2, wherein said Y is selected from a group consisting of naphthalene, phenathrene, pyrene and perylene.
[claim4]
4. The labeling agent according to claim 1, comprising a plurality of said nucleotide derivative units in a continuous series.
[claim5]
5. The labeling agent according to claim 1, comprising a plurality of said nucleotide derivative units, with linker units in between.
[claim6]
6. The labeling agent according to claim 1, wherein said multiple nucleotide units are two nucleotide derivative units in which said Y is the same or different, and are provided in a continuous series.
[claim7]
7. A set of labeling agents comprising two or more labeling agents each provided with a plurality of nucleotide derivative units represented by Formula (1) below:

(where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X1 represents O, S or Se, and X2 represents SH (or S-), Se-, or a C1-4 alkyl group or morpholino group), wherein the sequence of said Y in said plurality of nucleotide derivative units is different for each of the labeling agents.
[claim8]
8. The set of labeling agents according to claim 7, further comprising a labeling agent provided with one of the nucleotide derivative units represented by Formula (1) above.
[claim9]
9. An oligonucleotide probe comprising a plurality of nucleotide derivative units represented by Formula (1) below:

(where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X1 represents O, S or Se, and X2 represents SH (or S-), Se-, or a C1-4 alkyl group or morpholino group).
[claim10]
10. The oligonucleotide probe according to claim 9, comprising said plurality of nucleotide derivative units at the 3' terminal end or 5' terminal end.
[claim11]
11. An oligonucleotide probe set comprising two or more oligonucleotide probes each provided with a plurality of nucleotide derivative units represented by Formula (1) below:

(where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X1 represents O, S or Se, and X2 represents SH (or S-), Se- or a C1-4 alkyl group or morpholino group), wherein the sequence of said Y in said plurality of nucleotide derivative units is different for each of the probes.
[claim12]
12. The oligonucleotide probe set according to claim 11, further comprising a labeling agent provided with one of the nucleotide derivative units represented by Formula (1).
  • 発明者/出願人(英語)
  • UENO YOSHIHITO
  • KITADE YUKIO
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
米国特許分類/主・副
  • C09B001/00
  • C09B003/14
  • C09B057/00B
  • C09B069/10B
  • C09B069/10R
参考情報 (研究プロジェクト等) PRESTO RNA and biofunctions AREA
ライセンスをご希望の方、特許の内容に興味を持たれた方は、問合せボタンを押してください。

PAGE TOP

close
close
close
close
close
close