Oligonucleotide derivative, labeling agent and use for labeling agent
| Foreign code | F120006738 |
|---|---|
| File No. | K02704WO |
| Posted date | May 30, 2012 |
| Country | United States of America |
| Application number | 200913126320 |
| Gazette No. | 20120126175 |
| Gazette No. | 8354515 |
| Date of filing | Nov 2, 2009 |
| Gazette Date | May 24, 2012 |
| Gazette Date | Jan 15, 2013 |
| International application number | JP2009068785 |
| International publication number | WO2010055789 |
| Date of international filing | Nov 2, 2009 |
| Date of international publication | May 20, 2010 |
| Priority data |
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| Title | Oligonucleotide derivative, labeling agent and use for labeling agent |
| Abstract |
(US8354515) A labeling agent comprising a plurality of nucleotide derivative units represented by Formula (1) below: (where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group, X1 represents O, S or Se, and X2 represents SH (or S－), Se－, a C1-4 alkyl or a morpholino group). |
| Scope of claims |
[claim1] 1. A labeling agent comprising a plurality of nucleotide derivative units represented by Formula (1) below: (where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X1 represents O, S or Se, and X2 represents SH (or S-), Se-, or a C1-4 alkyl group or morpholino group). [claim2] 2. The labeling agent according to claim 1, wherein said Y is a condensed polycyclic hydrocarbon group. [claim3] 3. The labeling agent according to claim 2, wherein said Y is selected from a group consisting of naphthalene, phenathrene, pyrene and perylene. [claim4] 4. The labeling agent according to claim 1, comprising a plurality of said nucleotide derivative units in a continuous series. [claim5] 5. The labeling agent according to claim 1, comprising a plurality of said nucleotide derivative units, with linker units in between. [claim6] 6. The labeling agent according to claim 1, wherein said multiple nucleotide units are two nucleotide derivative units in which said Y is the same or different, and are provided in a continuous series. [claim7] 7. A set of labeling agents comprising two or more labeling agents each provided with a plurality of nucleotide derivative units represented by Formula (1) below: (where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X1 represents O, S or Se, and X2 represents SH (or S-), Se-, or a C1-4 alkyl group or morpholino group), wherein the sequence of said Y in said plurality of nucleotide derivative units is different for each of the labeling agents. [claim8] 8. The set of labeling agents according to claim 7, further comprising a labeling agent provided with one of the nucleotide derivative units represented by Formula (1) above. [claim9] 9. An oligonucleotide probe comprising a plurality of nucleotide derivative units represented by Formula (1) below: (where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X1 represents O, S or Se, and X2 represents SH (or S-), Se-, or a C1-4 alkyl group or morpholino group). [claim10] 10. The oligonucleotide probe according to claim 9, comprising said plurality of nucleotide derivative units at the 3' terminal end or 5' terminal end. [claim11] 11. An oligonucleotide probe set comprising two or more oligonucleotide probes each provided with a plurality of nucleotide derivative units represented by Formula (1) below: (where, Z represents a nitrogen atom or CH, Y represents an uncondensed aromatic hydrocarbon group or condensed polycyclic hydrocarbon group other than naturally occurring base having purine structure or pyrimidine structure, X1 represents O, S or Se, and X2 represents SH (or S-), Se- or a C1-4 alkyl group or morpholino group), wherein the sequence of said Y in said plurality of nucleotide derivative units is different for each of the probes. [claim12] 12. The oligonucleotide probe set according to claim 11, further comprising a labeling agent provided with one of the nucleotide derivative units represented by Formula (1). |
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| IPC(International Patent Classification) |
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| Reference ( R and D project ) | PRESTO RNA and biofunctions AREA |
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Contact Information for " Oligonucleotide derivative, labeling agent and use for labeling agent "
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- URL: http://www.jst.go.jp/chizai/
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