Top > Search of International Patents > MODIFIED FLUORESCENT PROTEIN

MODIFIED FLUORESCENT PROTEIN

Foreign code F120006797
File No. K02611WO
Posted date Jun 28, 2012
Country WIPO
International application number 2011JP075932
International publication number WO 2012063897
Date of international filing Nov 10, 2011
Date of international publication May 18, 2012
Priority data
  • P2010-254016 (Nov 12, 2010) JP
Title MODIFIED FLUORESCENT PROTEIN
Abstract Provided is a modified fluorescent protein which enables the detection of a power applied to a liquid where the fluorescent protein exists. A modified fluorescent protein, wherein a peptide linker is inserted into a position homologous to the position between the 144th and 145th amino acids in the amino acid sequence of a wild type fluorescent protein from jellyfish or a fluorescent protein derived from said wild type fluorescent protein, characterized in that the fluorescence properties of said modified fluorescent protein change depending on a change in a pressure that is applied to a liquid where said modified fluorescent protein exists.
Outline of related art and contending technology BACKGROUND ART
Fluorescent protein, for example 6 amino acid sequence represented by the sequence set forth in the green fluorescent protein, in basic research and applied research, usually, used as a material of fluorescently labeling a protein. By the fluorescent label is a fluorescent protein, under an optical microscope, the localization of the protein can be observed and exercise. In addition, be genetically engineered can be added, such as pH or calcium concentration in the cytoplasm, environment-sensitive fluorescent proteins also can be made (Patent Document 1 and 2). Sensing environmental changes in the cytoplasm and the fluorescent protein can be, in order to examine the biological phenomenon, is a very powerful tool.
On the other hand, for a long time, the pressure in the cytoplasm, such as determining the tissue form during development, biological phenomena in vivo pressure is a strong correlation has been suggested.
Scope of claims (In Japanese)請求の範囲 [請求項1]
クラゲ由来の野生型蛍光蛋白質又は前記野生型蛍光蛋白質に由来する蛍光蛋白質のアミノ酸配列の144番目と145番目との間に相同する位置にペプチドリンカーが挿入されている改変蛍光蛋白質であって、前記改変蛍光蛋白質が存在する液体に加えられる圧力の変化に応じて前記改変蛍光蛋白質の蛍光特性が変化することを特徴とする改変蛍光蛋白質。

[請求項2]
前記ペプチドリンカーが、1~3個のアミノ酸である、請求項1記載の改変蛍光蛋白質。

[請求項3]
前記ペプチドリンカーのアミノ酸が、グリシンを含む、請求項1又は2記載の改変蛍光蛋白質。

[請求項4]
前記野生型蛍光蛋白質が、全長238アミノ酸残基の緑色蛍光蛋白質(GFP)である、請求項1から3のいずれかに記載の改変蛍光蛋白質。

[請求項5]
クラゲ由来の蛍光蛋白質を原料とするものである、請求項1から4のいずれかに記載の改変蛍光蛋白質。

[請求項6]
前記野生型蛍光蛋白質に由来する蛍光蛋白質が、YFP、CFP、EGFP、EYFP、及びECFPからなる群から選択される、請求項1から5のいずれかに記載の改変蛍光蛋白質。

[請求項7]
請求項1から6のいずれかに記載の改変蛍光蛋白質と、前記改変蛍光蛋白質の励起スペクトルとは異なる励起スペクトルの蛍光蛋白質とが融合した融合蛍光蛋白質。

[請求項8]
請求項1から6のいずれかに記載の改変蛍光蛋白質又は請求項7記載の融合蛍光蛋白質をコードするベクター。

[請求項9]
請求項1から6のいずれかに記載の改変蛍光蛋白質又は請求項7記載の融合蛍光蛋白質が遺伝子導入された細胞。

[請求項10]
請求項1から6のいずれかに記載の改変蛍光蛋白質又は請求項7記載の融合蛍光蛋白質が遺伝子導入されたヒトを除く生物。

[請求項11]
請求項1から6のいずれかに記載の改変蛍光蛋白質又は請求項7記載の融合蛍光蛋白質が存在する液体にかかる圧力又は圧力変化を検出する方法であって、前記改変蛍光蛋白質又は融合蛍光蛋白質の蛍光強度又は蛍光波長を検出することを含む、方法。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • Inventor
  • WATANABE Tomonobu
  • YOSHIZAWA Keiko
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS JP KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
Reference ( R and D project ) PRESTO Life Phenomena and Measurement Analysis AREA
Please contact us by E-mail or facsimile if you have any interests on this patent.

PAGE TOP

close
close
close
close
close
close