KIT FOR DETECTION OF GENE TARGETED BY MICRORNA, AND METHOD FOR DETECTION OF GENE TARGETED BY MICRORNA
Disclosed are: a detection kit for detecting a gene targeted by miRNA
and a method for detecting a gene targeted by miRNA in a simple manner without the need of carrying out any operation for introducing a gene into a cell using a vector. Specifically disclosed is a kit for detecting a gene targeted by microRNA, which comprises a cell extraction reagent, a microRNA-labeling reagent or labeled microRNA, and a reaction reagent with the microRNA-labeling substance. A cell extract is prepared under mild conditions, labeled miRNA is added to the cell extract, and a labeled substance is collected. In this manner, mRNA for a gene targeted by miRNA can be collected readily. cDNA is produced from the collected mRNA, and a gene targeted by miRNA is detected using the cDNA.