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KIT FOR DETECTION OF GENE TARGETED BY MICRORNA, AND METHOD FOR DETECTION OF GENE TARGETED BY MICRORNA

Foreign code F120006853
File No. S2010-0909-C0
Posted date Sep 19, 2012
Country WIPO
International application number 2011JP063636
International publication number WO 2011/158847
Date of international filing Jun 15, 2011
Date of international publication Dec 22, 2011
Priority data
  • P2010-137924 (Jun 17, 2010) JP
Title KIT FOR DETECTION OF GENE TARGETED BY MICRORNA, AND METHOD FOR DETECTION OF GENE TARGETED BY MICRORNA
Abstract

Disclosed are: a detection kit for detecting a gene targeted by miRNA

and a method for detecting a gene targeted by miRNA in a simple manner without the need of carrying out any operation for introducing a gene into a cell using a vector. Specifically disclosed is a kit for detecting a gene targeted by microRNA, which comprises a cell extraction reagent, a microRNA-labeling reagent or labeled microRNA, and a reaction reagent with the microRNA-labeling substance. A cell extract is prepared under mild conditions, labeled miRNA is added to the cell extract, and a labeled substance is collected. In this manner, mRNA for a gene targeted by miRNA can be collected readily. cDNA is produced from the collected mRNA, and a gene targeted by miRNA is detected using the cDNA.

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NATIONAL UNIVERSITY CORPORATION OKAYAMA UNIVERSITY,
  • OUCHIDA, MAMORU,
  • ITO, SACHIO
  • Inventor
  • OUCHIDA, MAMORU,
  • ITO, SACHIO
IPC(International Patent Classification)

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