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ANTIBODY PRODUCTION METHOD

外国特許コード F120006894
整理番号 S2011-0275
掲載日 2012年10月3日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2012JP056691
国際公開番号 WO 2012124764
国際出願日 平成24年3月15日(2012.3.15)
国際公開日 平成24年9月20日(2012.9.20)
優先権データ
  • 特願2011-059739 (2011.3.17) JP
発明の名称 (英語) ANTIBODY PRODUCTION METHOD
発明の概要(英語) Provided is an antibody production method whereby it is possible to repeatedly acquire antibodies produced by fish without killing the fish. Specifically provided is an antibody production method whereby it is possible to repeatedly acquire the antibodies produced by a fish, without killing the fish, by administering antigens to fish that have blisters.
従来技術、競合技術の概要(英語) BACKGROUND ART
Current, E. coli by genetic recombination technique, and the proteins useful in the production or the like and, further, this as an antigen by administering to a mouse or the like, a technique for the production of antibodies are widely used. However, such as E. coli protein is produced in prokaryotic organisms, multimer formation, post-translational modification such as glycosylation has not been performed accurately. In addition, a complete protein yeast produced has not been performed for post-translational modification, in some cases may not have a function of the original problem.
In order to solve these problems, and insect cells, mouse, pig, useful in mammals such as human cells of the animal to produce the protein technology is used, the cost of the for culturing cells, time to obtain the useful protein is such a problem. Therefore, in recent years, make use of a fish as the bioreactor has attracted attention. Proteins from fish, multimer formation in the same manner as a mammal, such as glycosylation is the advantage that the post-translational modification. By utilizing the advantages in Patent Document 1 therefore, transgenic fish of the multimeric glycoproteins can be produced, embryo, larvae, fry or the like tissue, from blood recovered from disclosed.
Rearing fish is relatively easy, cost is low is maintained. In addition, water or food, duration of sunshine, such as egg laying and approximately about water quality can be controlled by, an advantage of having a very easily handled. The inventors of the present invention, antigen-displaying yeast is created, which as a feed to the fish such as zebrafish by providing, from fish and a method for producing specific antibodies, conventional, has been difficult (g protein-coupled receptor; GPCR) GPCR such as the production of antibodies against membrane proteins to the fish or the like (Japanese Patent Application 2009-83900), by a variety of techniques developed for fish (for example, Patent Document 2, 3, reference). However, these techniques have, for recovery of the antibody from the whole fish, fish in a state where the making, produced antibodies cannot be repeatedly obtained.
The inventors of the present invention, in order to solve this problem, in the development of the present invention, such as fish eye having a water bubble with attention paid to bubbles. As this type of goldfish is ornamental, raised for the widely and overseas. In Patent Document 4, liquid water voids (bubbles) in the blistering of the eye, cell growth-promoting or cytoprotective of fish cells to act, in processing operations such as fish cells, unfertilized egg egg quality while maintaining effective operation possible with the disclosed. However, this patent, blisters (bubbles) in the solution as an antibody useful to produce the protein or the like is not suggested, also in the prior art, to produce an antibody having fish bubbles attempt has not been used.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • National University Corporation Mie University
  • 発明者(英語)
  • TAMARU, Yutaka
  • TSUTSUMI, Hiroko
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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