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ANTIBODY PRODUCTION METHOD

Foreign code F120006894
File No. S2011-0275
Posted date Oct 3, 2012
Country WIPO
International application number 2012JP056691
International publication number WO 2012124764
Date of international filing Mar 15, 2012
Date of international publication Sep 20, 2012
Priority data
  • P2011-059739 (Mar 17, 2011) JP
Title ANTIBODY PRODUCTION METHOD
Abstract Provided is an antibody production method whereby it is possible to repeatedly acquire antibodies produced by fish without killing the fish. Specifically provided is an antibody production method whereby it is possible to repeatedly acquire the antibodies produced by a fish, without killing the fish, by administering antigens to fish that have blisters.
Outline of related art and contending technology BACKGROUND ART
Current, E. coli by genetic recombination technique, and the proteins useful in the production or the like and, further, this as an antigen by administering to a mouse or the like, a technique for the production of antibodies are widely used. However, such as E. coli protein is produced in prokaryotic organisms, multimer formation, post-translational modification such as glycosylation has not been performed accurately. In addition, a complete protein yeast produced has not been performed for post-translational modification, in some cases may not have a function of the original problem.
In order to solve these problems, and insect cells, mouse, pig, useful in mammals such as human cells of the animal to produce the protein technology is used, the cost of the for culturing cells, time to obtain the useful protein is such a problem. Therefore, in recent years, make use of a fish as the bioreactor has attracted attention. Proteins from fish, multimer formation in the same manner as a mammal, such as glycosylation is the advantage that the post-translational modification. By utilizing the advantages in Patent Document 1 therefore, transgenic fish of the multimeric glycoproteins can be produced, embryo, larvae, fry or the like tissue, from blood recovered from disclosed.
Rearing fish is relatively easy, cost is low is maintained. In addition, water or food, duration of sunshine, such as egg laying and approximately about water quality can be controlled by, an advantage of having a very easily handled. The inventors of the present invention, antigen-displaying yeast is created, which as a feed to the fish such as zebrafish by providing, from fish and a method for producing specific antibodies, conventional, has been difficult (g protein-coupled receptor; GPCR) GPCR such as the production of antibodies against membrane proteins to the fish or the like (Japanese Patent Application 2009-83900), by a variety of techniques developed for fish (for example, Patent Document 2, 3, reference). However, these techniques have, for recovery of the antibody from the whole fish, fish in a state where the making, produced antibodies cannot be repeatedly obtained.
The inventors of the present invention, in order to solve this problem, in the development of the present invention, such as fish eye having a water bubble with attention paid to bubbles. As this type of goldfish is ornamental, raised for the widely and overseas. In Patent Document 4, liquid water voids (bubbles) in the blistering of the eye, cell growth-promoting or cytoprotective of fish cells to act, in processing operations such as fish cells, unfertilized egg egg quality while maintaining effective operation possible with the disclosed. However, this patent, blisters (bubbles) in the solution as an antibody useful to produce the protein or the like is not suggested, also in the prior art, to produce an antibody having fish bubbles attempt has not been used.
Scope of claims (In Japanese)請求の範囲 [請求項1]
水泡を有する魚類に抗原を投与し、抗体を産生させる工程を含む、抗体の製造方法。

[請求項2]
抗原を投与する部位が水泡である請求項1に記載の製造方法。

[請求項3]
抗原をオイルベースとともに、またはオイルベースと不活化大腸菌とともに投与する請求項1または2に記載の製造方法。

[請求項4]
魚類に産生させた抗体を、魚類の水泡から採取する工程を含む、請求項1~3のいずれかに記載の製造方法。

[請求項5]
水泡を有する魚類が水泡眼またはらんちゅうである請求項1~4のいずれかに記載の製造方法。

[請求項6]
抗原がタンパク質または糖タンパク質である請求項1~5のいずれかに記載の製造方法。

[請求項7]
抗原がEGFP、グルコアミラーゼまたはLGR3である請求項6に記載の製造方法。

[請求項8]
請求項1~7のいずれかに記載の製造方法によって製造される抗体。

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • National University Corporation Mie University
  • Inventor
  • TAMARU, Yutaka
  • TSUTSUMI, Hiroko
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IS KE KG KM KN KP KR KZ LA LC LK LR LS LT LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PE PG PH PL PT QA RO RS RU RW SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ MD RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG
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