GERM CELL MARKER USING FISH VASA GENE
外国特許コード | F120006912 |
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整理番号 | S2008-0954 |
掲載日 | 2012年10月9日 |
出願国 | 世界知的所有権機関(WIPO) |
国際出願番号 | 2008JP000743 |
国際公開番号 | WO 2008/129838 |
国際出願日 | 平成20年3月26日(2008.3.26) |
国際公開日 | 平成20年10月30日(2008.10.30) |
優先権データ |
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発明の名称 (英語) | GERM CELL MARKER USING FISH VASA GENE |
発明の概要(英語) |
For examining whether or not a germ cell derived from a donor fish, which is transplanted into a heterogeneous recipient fish by surrogate fish technique, is grown and matured in a gonad of the recipient fish, it is required to utilize, as an indicator, a trait which is expressed specifically in the germ cell and can be used for distinguishing the recipient fish from the donor fish. Vasa gene, which is a gene specific to a germ cell, is specific to a primordial germ cell and/or a spermatogenous cell/oogonium, and the expression of Vasa gene is not observed in a somatic cell. The sequences of Vasa genes of a tuna, a chub mackerel, a spotted mackerel, an eastern little tuna and a drumfish are determined, and the expression of each of the genes is utilized as a maker for a germ cell. It is possible to detect only a tuna Vasa gene specifically from Vasa gene sequences which are highly conserved in fishes, without the need of sequencing. Therefore, it is possible to identify a tuna-derived germ cell in a gonad of a recipient fish in a simple and reliable manner. Thus, a tuna fish can be grown and bred with good efficiency. By utilizing the above-mentioned knowledge, it becomes possible to detect a germ cell derived from a donor fish from a germ cell derived from a heterogeneous recipient fish with good efficiency even when, besides a tuna fish, a fish belonging to the order Perciformes is used as a donor fish. |
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国際特許分類(IPC) |
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日本語項目の表示
発明の名称 | 魚類のVasa遺伝子を用いた生殖細胞マーカー |
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