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GERM CELL MARKER USING FISH VASA GENE

Foreign code F120006912
File No. S2008-0954
Posted date Oct 9, 2012
Country WIPO
International application number 2008JP000743
International publication number WO 2008/129838
Date of international filing Mar 26, 2008
Date of international publication Oct 30, 2008
Priority data
  • P2007-080022 (Mar 26, 2007) JP
Title GERM CELL MARKER USING FISH VASA GENE
Abstract

For examining whether or not a germ cell derived from a donor fish, which is transplanted into a heterogeneous recipient fish by surrogate fish technique, is grown and matured in a gonad of the recipient fish, it is required to utilize, as an indicator, a trait which is expressed specifically in the germ cell and can be used for distinguishing the recipient fish from the donor fish. Vasa gene, which is a gene specific to a germ cell, is specific to a primordial germ cell and/or a spermatogenous cell/oogonium, and the expression of Vasa gene is not observed in a somatic cell. The sequences of Vasa genes of a tuna, a chub mackerel, a spotted mackerel, an eastern little tuna and a drumfish are determined, and the expression of each of the genes is utilized as a maker for a germ cell. It is possible to detect only a tuna Vasa gene specifically from Vasa gene sequences which are highly conserved in fishes, without the need of sequencing. Therefore, it is possible to identify a tuna-derived germ cell in a gonad of a recipient fish in a simple and reliable manner. Thus, a tuna fish can be grown and bred with good efficiency. By utilizing the above-mentioned knowledge, it becomes possible to detect a germ cell derived from a donor fish from a germ cell derived from a heterogeneous recipient fish with good efficiency even when, besides a tuna fish, a fish belonging to the order Perciformes is used as a donor fish.

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY,
  • NAGASAKI PREFECTURAL GOVERNMENT,
  • NIPPON SUISAN KAISHA, LTD,
  • YOSHIZAKI, GORO,
  • TAKEUCHI, YUTAKA,
  • NAGASAWA, KAZUE,
  • HIGUCHI, KENTARO,
  • MORITA, TETSURO,
  • KABEYA, NAOKI
  • Inventor
  • YOSHIZAKI, GORO,
  • TAKEUCHI, YUTAKA,
  • NAGASAWA, KAZUE,
  • HIGUCHI, KENTARO,
  • MORITA, TETSURO,
  • KABEYA, NAOKI
IPC(International Patent Classification)

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