GERM CELL MARKER USING FISH VASA GENE
|Posted date||Oct 9, 2012|
|International application number||2008JP000743|
|International publication number||WO 2008/129838|
|Date of international filing||Mar 26, 2008|
|Date of international publication||Oct 30, 2008|
|Title||GERM CELL MARKER USING FISH VASA GENE|
For examining whether or not a germ cell derived from a donor fish, which is transplanted into a heterogeneous recipient fish by surrogate fish technique, is grown and matured in a gonad of the recipient fish, it is required to utilize, as an indicator, a trait which is expressed specifically in the germ cell and can be used for distinguishing the recipient fish from the donor fish. Vasa gene, which is a gene specific to a germ cell, is specific to a primordial germ cell and/or a spermatogenous cell/oogonium, and the expression of Vasa gene is not observed in a somatic cell. The sequences of Vasa genes of a tuna, a chub mackerel, a spotted mackerel, an eastern little tuna and a drumfish are determined, and the expression of each of the genes is utilized as a maker for a germ cell. It is possible to detect only a tuna Vasa gene specifically from Vasa gene sequences which are highly conserved in fishes, without the need of sequencing. Therefore, it is possible to identify a tuna-derived germ cell in a gonad of a recipient fish in a simple and reliable manner. Thus, a tuna fish can be grown and bred with good efficiency. By utilizing the above-mentioned knowledge, it becomes possible to detect a germ cell derived from a donor fish from a germ cell derived from a heterogeneous recipient fish with good efficiency even when, besides a tuna fish, a fish belonging to the order Perciformes is used as a donor fish.
|IPC(International Patent Classification)|
Contact Information for " GERM CELL MARKER USING FISH VASA GENE "
- Tokyo University of Marine Science and Technology (In Japanese)産学・地域連携推進機構担当
- URL: http://olcr.kaiyodai.ac.jp/
- Address: (In Japanese)〒108-8477 東京都港区港南4-5-7
- Fax: 03-5463-0894