Top > Search of International Patents > GENE MARKER LINKED TO GENETIC SEX OF YELLOWTAIL, SEX DETERMINATION METHOD FOR YELLOWTAIL, AND PRIMER FOR USE IN SEX DETERMINATION METHOD

GENE MARKER LINKED TO GENETIC SEX OF YELLOWTAIL, SEX DETERMINATION METHOD FOR YELLOWTAIL, AND PRIMER FOR USE IN SEX DETERMINATION METHOD

Foreign code F120006920
File No. S2009-0573
Posted date Oct 10, 2012
Country WIPO
International application number 2010JP055236
International publication number WO 2010/110373
Date of international filing Mar 25, 2010
Date of international publication Sep 30, 2010
Priority data
  • P2009-075492 (Mar 26, 2009) JP
Title GENE MARKER LINKED TO GENETIC SEX OF YELLOWTAIL, SEX DETERMINATION METHOD FOR YELLOWTAIL, AND PRIMER FOR USE IN SEX DETERMINATION METHOD
Abstract

Genomic DNA from a yellowtail is subjected to a PCR method or a nucleic acid amplification reaction method using an yellowtail-derived nucleic acid as a template, wherein the PCR method or the nucleic acid amplification reaction method is carried out using, as primers, oligonucleotides comprising the nucleotide sequences: 5'-TTTCATTGTGGCGCTCAG-3' and 5'-GGTTGTAATGTGTCCCAG-3' both of which are produced based on a gene marker for genetic linkage group (LG) 12 in an yellowtail genetic linkage map. A product of the method is subjected to a gel electrophoresis method to analyze the product. In this manner, the genetic sex of the yellowtail can be determined. This technique enables the determination of the genetic sex of an immature yellowtail with reliability without killing the yellowtail.

  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY,
  • INCORPORATED ADMINISTRATIVE AGENCY FISHERIES RESEARCH AGENCY,
  • SAKAMOTO, TAKASHI,
  • FUJI, KANAKO,
  • OZAKI, AKIYUKI,
  • OKAUCHI, MASANORI,
  • ARAKI, KAZUO,
  • HATTORI, KEITA,
  • YOSHIDA, KAZUNORI
  • Inventor
  • SAKAMOTO, TAKASHI,
  • FUJI, KANAKO,
  • OZAKI, AKIYUKI,
  • OKAUCHI, MASANORI,
  • ARAKI, KAZUO,
  • HATTORI, KEITA,
  • YOSHIDA, KAZUNORI
IPC(International Patent Classification)

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