METHOD FOR DETERMINING CANCER ONSET OR CANCER ONSET RISK
Provided is a method for highly accurately and quantitatively determining cancer onset or cancer onset risk by quantitative tissue-staining which is conducted in a biotissue with the use of an antibody capable of recognizing a cancer proliferation inhibitor or a cancer metastasis inhibitor, for example, PAR1 antibody inhibiting the mobility and infiltrating properties of cancer cells. Cancer onset or cancer onset risk is determined by a tissue staining method, said method comprising: a step for labeling an antibody, which is capable of recognizing a cancer proliferation inhibitor or a cancer metastasis inhibitor, with a fluorescent substance and then contacting the fluorescent-labeled antibody with a tissue sample
a step for irradiating a tissue site, which has been contacted with said antibody, with an excitation light to give a fluorescence image
a step for acquiring an intrinsic fluorescence image in a neighborhood region on a shorter or longer wavelength side, compared with the region wherein the wavelength of the fluorescence emitted by the fluorescent substance is acquired, in the same visual field at the same focus point as said fluorescence image
a step for conducting an image processing for removing the fluorescent brightness of said intrinsic fluorescence image from the fluorescent brightness of said fluorescence image to give a corrected fluorescence image
a step for counting cells at the tissue site which has been contacted with the antibody
a step for measuring the average fluorescent brightness per fluorescent particle
and a step for calculating the fluorescent particle count per cell.